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Biological Buffers

Light blue biological buffer solution in beaker with pipette.

It’s important to protect the integrity of your biomolecules and reagents by choosing the right biological buffer systems. We offer an extensive portfolio of biological buffers for a wide variety of applications, including cell culture buffers, PCR buffers, HEPES buffers, and assay buffers.

Key features of our advanced buffer systems:

  • High-purity solutions available in a wide range of grades
  • Multiple and user-friendly packaging options
  • Assured solution stability with pH control
  • No interference with biological processes
  • Add critical salts and nutrients to cells and tissues
  • Scalable from early research to commercial applications
  • Customized blending

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Trizma<SUP>®</SUP> base
T1503

Trizma® base

≥99.9% (titration), crystalline, primary standard, aminopeptidase substrate

Triethylammonium acetate buffer
90358

Triethylammonium acetate buffer

volatile buffer, ~1.0 M in H2O

Trizma<SUP>®</SUP> base
T6066

Trizma® base

BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

Trizma<SUP>®</SUP> hydrochloride
T3253

Trizma® hydrochloride

reagent grade, ≥99.0% (titration), crystalline

Imidazole
I202

Imidazole

ReagentPlus®, 99%

Dulbecco′s Phosphate Buffered Saline
D8662

Dulbecco′s Phosphate Buffered Saline

Liquid, with MgCl₂ and CaCl₂, sterile-filtered, suitable for cell culture

Dulbecco′s Phosphate Buffered Saline
D5652

Dulbecco′s Phosphate Buffered Saline

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

MES hydrate
M2933

MES hydrate

BioPerformance Certified, suitable for cell culture, ≥99.5%

Phosphate Buffered Saline with 0.05% TWEEN<SUP>®</SUP> 20, pH 7.4
P3563

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween® 20

Red Blood Cell Lysis Buffer
11814389001

Red Blood Cell Lysis Buffer

solution, Roche, pkg of 100 mL, sufficient for 50-500 reactions

di-Sodium hydrogen phosphate dihydrate
71643

di-Sodium hydrogen phosphate dihydrate

BioUltra, Molecular Biology, ≥99.0% (T)

Triethanolamine
90279

Triethanolamine

≥99.0% (GC)

Triethylammonium bicarbonate buffer
T7408

Triethylammonium bicarbonate buffer

1.0 M, pH 8.5±0.1

Sodium acetate buffer solution
S7899

Sodium acetate buffer solution

pH 5.2±0.1 (25 °C), Molecular Biology, 3 M, 0.2 μm filtered

Trizma<SUP>®</SUP> hydrochloride
T5941

Trizma® hydrochloride

BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Tromethamine
T6687

Tris Base

99-101% dry basis, crystalline, meets USP testing specifications

Phosphate buffered saline
P5493

Phosphate buffered saline

10X, solution, pH 7.2-7.6 (25 °C), BioPerformance Certified, for molecular biology

Phosphoric acid
49685

Phosphoric acid

suitable for HPLC, LiChropur, 85%

Tris base
TRIS-RO

Tris base

99.9% (titration), solid, abs., 100 mg/mL, 300 nm ≤0.015

Tris(hydroxymethyl)aminomethane
1.08382

Tris Base

solid, ACS reagent, reag. Ph. Eur., GR for analysis buffer substance


Our advanced buffering systems can bring phenomenal stability to your cell culture, polymerase chain reaction (PCR), drug screening, bioprocessing, purification, and final formulation-based applications. All our products can be scaled from early research to commercial applications.

Related Resources

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Complete Workflow Solutions

We provide a comprehensive selection of high-quality biochemicals in a wide range of buffer grades and innovative, user-friendly packaging options. In addition to off-the-shelf products, we offer custom reagent manufacturing and customized blending.

Bioperformance Certified Buffers

Our superior BioPerformance Certified buffers are stringently tested and pre-qualified to expedite your bioprocessing workflows in critical life science applications, such as cell culture, molecular biology, and diagnostic manufacturing. This may include enzymatic assay of nuclease, protease, and phosphatase, cellular toxicity, or endotoxin and bioburden levels. Using a single high-quality buffer across multiple workflows ensures the reproducibility of your experiment.

pHast Pack™ Ready-to-Use Buffers

Trying to get the correct concentration and pH value in buffer preparations can be a tedious and time-consuming task. Our pHast Pack™ ready-to-use buffers are specially designed to simplify your work. Simply mix the precisely pre-weighed packets with distilled water for quick and reproducible results again and again. By saving time, effort and even shelf space, you can focus on more important areas of your research endeavors.

Need help making a buffer? Our buffer preparation calculator helps you to quickly calculate buffer molarity, mass, and volume.





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