04-784

Anti-IRS1 Antibody, clone AW58, rabbit monoclonal

culture supernatant, clone AW58, Upstate^®

• 동의어(들): insulin receptor substrate 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 결합: unconjugated
• 항체 형태: culture supernatant
• 항체 생산 유형: primary antibodies
• 클론: AW58, monoclonal
• 종 반응성: human, rat, mouse
• 제조업체/상표: Upstate^®
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NM_005544
• UniProt 수납 번호: P35568
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... IRS1(3667)

설명

일반 설명

Insulin Receptor substrate proteins are the effectors of both Insulin and IGF-initiated signaling. They share PH and PTB domains near their n-termini, and multiple Tyr phosphorylation motifs in their c-terminal regions. Proteins which bind to tyrosine-phosphorylated IRS-proteins include PI3 Kinase p85, GRB2, SHP2, Nck, Crk, and Fyn. IRS1 appears to be principally involved in IGF-signaling and cytoskeletal growth. IRS2 appears to be the critical mediator of Insulin signaling, as genetic ablation results in type II diabetes. IRS3 is expressed primarily in adipocytes and is an unusually potent activator of PI3 Kinase. IRS4 lacks the tyrosine residues which in the other IRS-proteins binds to SHP2.

Mr ~145-165kDa

면역원

Peptide corresponding to carboxy-terminal 14 amino acids (C-YASINFQKQPEDRQ) of rat liver IRS1

애플리케이션

Anti-IRS1 Antibody, clone AW58 detects level of IRS1 & has been published & validated for use in IP & WB.

Immunoblot Analysis: 1:1,000 to 1:4,000 dilutions of this lot detected IRS1 in RIPA lysates from 3T3/A31 mouse fibroblasts.

Immunoprecipitation: 5-10μl of this lot immunoprecipitated IRS1 from 500μg of 3T3/A31 RIPA lysate.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

생화학적/생리학적 작용

IRS1

물리적 형태

Cultured supernantant in 0.05% sodium azide

제조 메모

1 year at -20°C from date of shipment

분석 메모

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot on in RIPA lysates from 3T3/A31 mouse fibroblasts

기타 정보

Replaces: 05-784

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable