05-1087

Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2

clone 24.6.2, from mouse

• 동의어(들): insulin receptor substrate 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 24.6.2, monoclonal
• 종 반응성: bovine, pig, mouse, monkey, canine, rat, human
• 기술: immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_005535.1
• UniProt 수납 번호: P35568
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer307 )
• 유전자 정보: bovine ... Irs1(538598) ; dog ... Irs1(486148) ; human ... IRS1(3667) ; mouse ... Irs1(16367) ; pig ... Irs1(100512686) ; rat ... Irs1(25467) ; rhesus monkey ... Irs1(707870)

설명

일반 설명

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.

~185 kDa

면역원

Synthetic peptide corresponding to amino acids surrounding phosphorylated Ser307 of mouse IRS1.

애플리케이션

This Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2 is validated for use in WB, IP, IC, IF for the detection of phospho-IRS1 (Ser307 mouse/ Ser312 human).

생화학적/생리학적 작용

Predicted to cross-react with many other species based on 100% sequence homology with immunogen.

This antibody recognizes IRS1 phosphorylated at Ser307 (mouse) and Ser312 (human).

물리적 형태

Format: Purified

분석 메모

Evaluated by western blot on IR/IRS1 transfected CHO +/- Insulin lysate.

Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect IRS1 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 07-247

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable