06-519-AF488

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor^™ 488 conjugate

from rabbit, ALEXA FLUOR^™ 647

• 동의어(들): Cyclic AMP-responsive element-binding protein 1 (pSer133), cAMP-responsive element-binding protein 1 (pSer133), CREB-1 (pSer133), CREB-A (pSer133), htCREB (pSer133), CREB-B (pSer119)
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 결합: ALEXA FLUOR^™ 647
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, rat, mouse
• 종 반응성(상동성에 의해 예측): Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology), chicken (based on 100% sequence homology), porcine (based on 100% sequence homology), guinea pig (based on 100% sequence homology)
• 기술: immunofluorescence: suitable
• 동형: IgG1
• NCBI 수납 번호: NP_604391
• UniProt 수납 번호: P16220
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer133)
• 유전자 정보: human ... CREB1(1385)

설명

일반 설명

Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation by various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.

~43 kDa observed.

면역원

Epitope: pSer133.

KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB-A.

애플리케이션

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor^™ 488 conjugate is an antibody against phospho-CREB for use in Immunofluorescence.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

The unconjugated antibody (Cat. No. 06-519) is shown to be suitable also for Electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry, and Western blotting applications.

생화학적/생리학적 작용

Recognizes phosphorylated CREB-A (pSer133), htCREB (pSer133), and CREB-B (pSer119) spliced isoforms reported by (UniProt P16220). May also recognize phosphorylated CREM and ATF-1 that contain homologous sequence to that of the immungen peptide.

물리적 형태

Affinity purified

Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

제조 메모

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Evaluated by Immunofluorescence in rat brain tissue.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected phospho-CREB (Ser133) in rat brain tissue.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable