To prepare this product, add 500µl of ultrapure water to make a 1mg/ml stock solution. It is recommended to aliquot the solution to avoid repeated freezing and thawing. The reconstituted solution can be stored for up to 6 months at -20°C. This product is suitable for use in immunohistochemistry at a 1:1000-1:4000 dilution. You can review available Certificates of Analysis by visiting the documentation section on our website.
생물학적 소스
rabbit
Quality Level
결합
peroxidase conjugate
항체 형태
affinity purified immunoglobulin
항체 생산 유형
secondary antibodies
클론
polyclonal
종 반응성
sheep
제조업체/상표
Upstate®
기술
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
배송 상태
wet ice
타겟 번역 후 변형
unmodified
1 of 4
이 품목 | 12-349 | AP180P | AP124C |
|---|---|---|---|
| species reactivity sheep | species reactivity mouse | species reactivity goat | species reactivity mouse |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate CY3 conjugate |
| biological source rabbit | biological source goat | biological source donkey | biological source goat |
| antibody form affinity purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form F(ab′)2 fragment of affinity isolated antibody |
| technique(s) ELISA: suitable, western blot: suitable, immunohistochemistry: suitable | technique(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable | technique(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable | technique(s) immunofluorescence: suitable |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
일반 설명
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
애플리케이션
Rabbit anti-Sheep IgG Antibody, HRP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA, WB, IH.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
물리적 형태
0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10mg/ml BSA, and 0.01% gentamicin sulfate
제조 메모
Lyophilized: 2 years at 4°C; Rehydrated: 6 months at -20°C.
분석 메모
Routinely evaluated by western blot
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Naveen Sharma et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 303(12), R1261-R1267 (2012-11-02)
Akt is a serine/threonine kinase that plays a key role in numerous cellular functions including metabolism, growth, protein synthesis, apoptosis, and cell proliferation. The most consistent and robust effect of moderate calorie restriction (CR; ~60% of ad libitum, AL, food
Salah Boudjadi et al.
Cancers, 9(8) (2017-09-22)
Integrins are a family of heterodimeric glycoproteins involved in bidirectional cell signaling that participate in the regulation of cell shape, adhesion, migration, survival and proliferation. The integrin α1β1 is known to be involved in RAS/ERK proliferative pathway activation and plays
Naveen Sharma et al.
PloS one, 8(6), e65118-e65118 (2013-06-12)
Calorie restriction (CR) (consuming ~60% of ad libitum, AL, intake) improves whole body insulin sensitivity and enhances insulin-stimulated glucose uptake by isolated skeletal muscles. However, little is known about CR-effects on in vivo glucose uptake and insulin signaling in muscle.
Arvin Bansal et al.
Journal of proteomics, 73(10), 2041-2049 (2010-07-06)
Exercise has been shown to improve function of the left ventricle (LV) following myocardial infarction (MI). The mechanisms to explain this benefit have not been fully delineated, but may involve improved mechanics resulting in unloading effects and increased endothelial nitric
Donel A Sequea et al.
Mechanisms of ageing and development, 134(1-2), 60-63 (2012-12-19)
Moderate calorie restriction (CR) can improve insulin-stimulated Akt phosphorylation and glucose uptake in muscles from 24 month-old rats, but the specific Akt substrates linking CR-effects on Akt to glucose uptake and other cellular processes are uncertain. We probed CR's influence
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Could you please provide the reconstitution method for this product and the recommended concentration for various uses?
1 답변-
도움이 되었습니까?
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What is the method of reconstitution for this product and what is the concentration recommended for various uses?
1 답변-
This product can be prepared by adding 500µl of ultrapure water to make a 1mg/ml stock solution. Aliquot to avoid repeated freezing and thawing. The reconstituted solution may be stored up to 6 months at -20°C. This product is tested for use in immunohistochemistry, at which a 1:1000-1:4000 dilution is suitable. See the link below to review available Certificates of Analysis: https://www.emdmillipore.com/product/Rabbit-Anti-Sheep-IgG-Antibody-HRP-conjugate,MM_NF-12-342#documentation
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