CC12

Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6)

liquid, clone DCS-6, Calbiochem^®

• 동의어(들): Anti-Bcl-1, Anti-PRAD-1
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: DCS-6, monoclonal
• 양식: liquid
• 포함: ≤0.1% sodium azide as preservative
• 종 반응성: human, mouse, rat
• 제조업체/상표: Calbiochem^®
• 저장 조건: do not freeze
• dilution: (Flow Cytometry (5 µg/mL); Frozen Sections ; Immunoblotting (1 µg/mL); Immunofluorescence (5 µg/mL); Immunoprecipitation ; Neutralization Studies ; Paraffin Sections (5 µg/mL, heat pre-treatment required))
• 동형: IgG2a
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CCND1(595) ; mouse ... Ccnd1(12443) ; rat ... Ccnd1(58919)

설명

일반 설명

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin D1 protein.

Recognizes cyclin D1 in MCF-7 and A431 cells. Does not react with cyclins D2 and D3.

This Anti-Cyclin D1 (Ab-3) Mouse mAb (DCS-6) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of Cyclin D1 (Ab-3).

면역원

Human

recombinant human cyclin D1

애플리케이션

Flow Cytometry (5 µg/ml; Cat. No. CC12, CC12F)

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (5 µg/ml; Cat. No. CC12, CC12F)

Immunoprecipitation (see application references)

Neutralization Studies (see application references)

Paraffin Sections (5 µg/ml, heat pre-treatment required)

포장

Please refer to vial label for lot-specific concentration.

물리적 형태

In 50 mM sodium phosphate buffer, 0.2% gelatin.

분석 메모

Negative Control
trp E (Ab-1)

Positive Control
MCF7, or A431 cells or breast carcinoma tissue

기타 정보

Does not react with cyclins D2 or D3. Staining of paraffin sections can also be detected with pepsin or trypsin pretreatment; light staining will be detected without pretreatment. Do not microwave sections, as this may destroy staining. For immunofluorescence and flow cytometry, we recommend using the FITC conjugate (Cat. No. CC12F). Antibody should be titrated for optimal results in individual systems.

Inaba, T., et al. 1992. Genomics13, 565.
Jiang, W., et al. 1992. Cancer Res.2, 2980.
Pines, J. 1992. Cell Growth Differ.2, 305.
Xiong, Y., et al. 1992. Genomics13, 575.
Xiong, Y., et al. 1992. Cell71, 504.
Motokura, T., et al. 1991. Nature350, 512.
Solomon, M.J., et al. 1990. Cell63, 1013.
Draetta, G., and Beach, D. 1988. Cell54, 17.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Toxicity: Standard Handling (A)

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable