GR01

Anti-EGFR (Ab-1) Mouse mAb (528)

liquid, clone 528, Calbiochem^®

• 동의어(들): Anti-Epidermal Growth Factor Receptor
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 528, monoclonal
• 양식: liquid
• 포함: ≤0.1% sodium azide as preservative
• 종 반응성: human
• 반응하면 안 됨: mouse, rat
• 제조업체/상표: Calbiochem^®
• 저장 조건: do not freeze
• dilution: (Flow Cytometry (5 µg/mL); Frozen Sections (2.5 µg/mL); Immunofluorescence (5 µg/mL); Immunoprecipitation(1 µg/sample); Neutralization Studies ; Paraffin Sections )
• 동형: IgG2a
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... EGFR(1956) ; mouse ... Egfr(13649) ; rat ... Egf(25313)

설명

일반 설명

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references. Recognizes the ~170 kDa EGF receptor.

Recognizes the ~170 kDa EGF receptor protein in A431 cells and normal skin.

애플리케이션

Flow Cytometry (5 µg/ml)

Frozen Sections (2.5 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation(1 µg/sample)

Neutralization Studies (see comments and application references)

Paraffin Sections (see application references)

포장

Please refer to vial label for lot-specific concentration.

분석 메모

Negative Control
HL-60 cells or human brain

Positive Control
A-431 or normal skin tissue

기타 정보

Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.

This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity (see application references). Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Toxicity: Standard Handling (A)

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable