QIA127

Rapid Cell Proliferation Kit

• 동의어(들): Proliferation assay kit
• UNSPSC 코드: 12352207
• NACRES: NA.84

속성

• 사용: sufficient for 500 tests
• Quality Level: 100
• 포장: pkg of 1 96-well plate(s)
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• 입력: sample type cultured cells (1,000-50,000 cells)
• 검출 방법: colorimetric
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.

생화학적/생리학적 작용

The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.

특징 및 장점

Assay Time: 0.5 - 4 h

Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)

제조 메모

• WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.

기타 정보

Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.

WST-1, 1-Methoxy PMS, and a user protocol.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

안전성 정보

• Storage Class Code: 10 - Combustible liquids