The binding target of this product is the f-peptide.
제품 이름
NISTmAb, Humanized IgG1k Monoclonal Antibody, NIST® SRM® 8671
antibody product type
primary antibodies
grade
certified reference material
form
liquid
manufacturer/tradename
NIST®
technique(s)
UV/Vis spectroscopy: suitable
liquid chromatography (LC): suitable
mass spectrometry (MS): suitable
format
matrix material
1 of 4
이 품목 | R8779 | NISTRM8398 | NIST3667 |
|---|---|---|---|
| manufacturer/tradename NIST® | manufacturer/tradename - | manufacturer/tradename NIST® | manufacturer/tradename NIST® |
| grade certified reference material | grade - | grade certified reference material | grade certified reference material |
| form liquid | form buffered aqueous solution | form liquid | form liquid |
| format matrix material | format - | format - | format matrix material |
| antibody product type primary antibodies | antibody product type primary antibodies | antibody product type - | antibody product type - |
| technique(s) UV/Vis spectroscopy: suitable | technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1 μg/mL using HeLa total cell extract | technique(s) - | technique(s) LC/MS: suitable |
Analysis Note
NIST8671 is intended for research use. It is not intended for animal or human consumption, clinical testing or therapeutic use.
Application
NISTmAb, Humanized IgG1k Monoclonal Antibody has been used as a standard for testing de novo sequencing algorithms on bottom-up mass spectrometry.[1]This reference material (RM) is primarily intended for evaluating the performance of methods for determining physiochemical and biophysical attributes of monoclonal antibodies. It also provides a representative test molecule for developing novel technology in therapeutic protein characterization. Other potential uses include:
- system suitability tests
- establishing method or instrument performance and variability
- comparing changing analytical test methods
- assisting in method qualification
General description
NISTmAb, Humanized IgG1k Monoclonal Antibody is a recombinant antibody expressed in murine suspension culture. It is a homodimer with two identical light chains and two identical heavy chains linked through inter- and intra- chain disulfide bonds with a molecular weight of 150 kDa. The protein possesses a low abundance of post-translational modifications including methionine oxidation, deamidation, and glycation. This humanized monoclonal antibody also has N-terminal pyroglutamination, C-terminal lysine clipping, and glycosylation of the heavy chains.
A unit of RM 8671 consists of one internal-threaded polypropylene cryovial containing 800 µL of 10 mg/mL NIST IgG1κ monoclonal antibody (NISTmAb) in 12.5 mmol/L L-histidine, 12.5 mmol/L L-histidine HCl (pH 6.0).
SRM 8671_Cert
SRM 8671_SDS
A unit of RM 8671 consists of one internal-threaded polypropylene cryovial containing 800 µL of 10 mg/mL NIST IgG1κ monoclonal antibody (NISTmAb) in 12.5 mmol/L L-histidine, 12.5 mmol/L L-histidine HCl (pH 6.0).
SRM 8671_Cert
SRM 8671_SDS
Other Notes
- RM 8671 is intended for research use.
- Details on expiration, storage, safety, usage, and source are provided in the NIST certificate.
- Information on biomaterials, disposal, and transport is available in the SDS.
Legal Information
NIST is a registered trademark of National Institute of Standards and Technology
SRM is a registered trademark of National Institute of Standards and Technology
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당사의 제품 선택기 도구.을(를) 시도해 보세요.
related product
저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Ioannis Karageorgos et al.
Biologicals : journal of the International Association of Biological Standardization, 50, 27-34 (2017-10-03)
Monoclonal antibody pharmaceuticals are the fastest-growing class of therapeutics, with a wide range of clinical applications. To assure their safety, these protein drugs must demonstrate highly consistent purity and stability. Key to these objectives is higher order structure measurements validated
Automated Antibody De Novo Sequencing and Its Utility in Biopharmaceutical Discovery.
Sen K I, et al.
Journal of the American Society For Mass Spectrometry, 28(5), 803-810 (2017)
문서
Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.
Learn about the separation of several charge variants of two monoclonal antibodies, NISTmAb and Infliximab, using a polymeric cation exchange column and MS-compatible mobile phases.
An optimized peptide mapping protocol using NISTmAb as a model monoclonal antibody, shorter incubation times, and improved digestion buffer to demonstrate minimal artificial asparagine deamidation and methionine oxidation.
This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.
관련 콘텐츠
Discover our wide variety of products for intact mass analysis of monoclonal antibodies, including size-exclusion columns (SEC), ion exchange columns, reverse-phase columns, HPLC buffers, MALDI matrices and standards, high-purity solvents, reagents, tools for protein sample preparation, and certified reference materials.
단클론 항체의 무손상 질량 분석을 위한 Merck의 다양한 제품에 대해 알아보세요. 크기 배제 컬럼(SEC, size-exclusion column), 이온 교환 컬럼, 역상 컬럼, HPLC 버퍼, MALDI 기질 및 표준 물질, 고순도 용매, 시약, 단백질 검체 준비용 기구, 공인 참조 물질 등이 있습니다.
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NISTｍAbの抗原は何ですか
1 답변-
도움이 되었습니까?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf도움이 되었습니까?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
도움이 되었습니까?
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How many mg are in 1 unit of NIST8671 - NISTmAb, Humanized IgG1k Monoclonal Antibody?
1 답변-
As mentioned in the 'DESCRIPTION' section, one unit or vial of RM 8671 contains 800 µL of 10 mg/mL NIST IgG1k monoclonal antibody (NISTmAb) in 12.5 mmol/L L-histidine, 12.5 mmol/L L-histidine HCl (pH 6.0).
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