A8811

Aldolase from rabbit muscle

Name: Aldolase from rabbit muscle
Brand: SIGMA
Price: 125048 KRW

ammonium sulfate suspension, 10-20 units/mg protein

동의어(들):

D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase, Fructose-diphosphate Aldolase

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크기 선택

200 UNITS

₩125,048

100 UNITS

₩128,195

1000 UNITS

₩556,322

5000 UNITS

₩1,905,561

₩125,048

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

9024-52-6

EC 번호:

4.1.2.13 (BRENDA, IUBMB)

MDL number:

MFCD00130453

UNSPSC 코드:

12352204

NACRES:

NA.54

주요 문서

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기술 서비스

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도움 문의

생물학적 소스

rabbit muscle

Quality Level

200

양식

ammonium sulfate suspension

특이 활성도

10-20 units/mg protein

외래 활성

glyceraldehyde-3-phosphate dehydrogenase ≤0.03%
lactic dehydrogenase ≤0.03%
phosphoglucose isomerase ≤0.6%
pyruvate kinase ≤0.1%
triosephosphate isomerase ≤0.05%

저장 온도

2-8°C

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차이점 표시

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이 품목	G6751	A2714	L2500
Sigma-Aldrich A8811 Aldolase from rabbit muscle	Sigma-Aldrich G6751 α-Glycerophosphate Dehydrogenase from rabbit muscle	Sigma-Aldrich A2714 Aldolase from rabbit muscle	Sigma-Aldrich L2500 L-Lactic Dehydrogenase from rabbit muscle
biological source rabbit muscle	biological source rabbit muscle	biological source rabbit muscle	biological source -
specific activity 10-20 units/mg protein	specific activity 100-300 units/mg protein	specific activity ≥8.0 units/mg protein	specific activity 800-1,200 units/mg protein
form ammonium sulfate suspension	form ammonium sulfate suspension	form lyophilized powder	form ammonium sulfate suspension
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
foreign activity glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, triosephosphate isomerase ≤0.05%, lactic dehydrogenase ≤0.03%, pyruvate kinase ≤0.1%	foreign activity Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%, Triosephosphate isomerase ≤0.02%	foreign activity glyceraldehyde-3-phosphate dehydrogenase ≤0.03%, lactic dehydrogenase ≤0.03%, phosphoglucose isomerase ≤0.6%, pyruvate kinase ≤0.1%, triosephosphate isomerase ≤0.05%	foreign activity pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 200

일반 설명

Aldolase exists as three isoforms in rabbit, which includes type A from muscle, type B from liver and brain associated type C. Aldolases correspond to a molecular weight of 158 kDa and exists as tetramer.[1]

애플리케이션

Aldolase from rabbit muscle has been used:

in standard 1-phosphofructokinase from rabbit muscle (RPFK-1) assay[2]
as a standard in the characterization of metabolic enzymes from glaucomatous tissues[3]
in fructose 2,6-bisphosphate assay of human cell lines[4]

Aldolase is used to convert fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Aldolase, from rabbit muscle has been used for stereospecific deprotonation at DHAP C3 [5].

생화학적/생리학적 작용

Aldolase interaction with Wiskott-Aldrich syndrome protein (WASP) may modulate actin dynamics.[6] It reverses the inhibition elicited by ascorbate on Muscle-type LDH (LDH-m4).[7]

Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β ⁸ barrel fold [5].

물리적 형태

Crystalline suspension in 2.5 M (NH₄)₂SO₄, 0.01 M Tris, pH 7.5, 0.001 M EDTA

분석 메모

Protein determined by biuret.

기타 정보

One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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시험 성적서(COA)

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문서 라이브러리 방문

Structure of rabbit muscle aldolase at low resolution.

Sygusch J, et al.

The Journal of Biological Chemistry, 260(28), 15286-15290 (1985)

Effect of lithium salts on lactate dehydrogenase, adenylate kinase, and 1-phosphofructokinase activities

Russell P, et al.

Journal of Enzyme Inhibition and Medicinal Chemistry, 25(4), 551-556 (2010)

Structure of a class I tagatose-1,6-bisphosphate aldolase: investigation into an apparent loss of stereospecificity.

Clotilde LowKam et al.

The Journal of biological chemistry, 285(27), 21143-21152 (2010-04-30)

Tagatose-1,6-bisphosphate aldolase from Streptococcus pyogenes is a class I aldolase that exhibits a remarkable lack of chiral discrimination with respect to the configuration of hydroxyl groups at both C3 and C4 positions. The enzyme catalyzes the reversible cleavage of four

A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome protein

St-Jean M, et al.

The Journal of biological chemistry, 282(19), 14309-14315 (2007)

Aldolase and actin protect rabbit muscle lactate dehydrogenase from ascorbate inhibition

Russell PJ, et al.

Journal of Enzyme Inhibition and Medicinal Chemistry, 19(1), 91-98 (2004)

모든 관련된 서류 보기

문서

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

질문

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활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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Aldolase from rabbit muscle