B5655

BCIP^®/NBT

Name: BCIP<SUP>&#174;</SUP>/NBT
Brand: SIGMA
Price: 99369 KRW
Availability: InStock

SIGMAFAST^™, alkaline phosphatase substrate, chromogenic, tablet

SIGMAFAST™ BCIP®/NBT

동의어(들):

BCIP/NBT Substrate, BCIP^®/NBT Alkaline Phosphatase Substrate

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5 TABS

₩99,369

25 TABS

₩400,621

₩99,369

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:

12352204

NACRES:

NA.83

주요 문서

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기술 서비스

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제품 이름

SIGMAFAST^™ BCIP^®/NBT, tablet

form

tablet

solubility

water: 10 mL, clear, yellow

storage temp.

−20°C

Quality Level

200

유사한 품목 비교

전체 비교 보기

차이점 표시

1 of 4

이 품목	D4418	P9187	B0274
Sigma-Aldrich B5655 SIGMAFAST^™ BCIP^®/NBT	Sigma-Aldrich D4418 SIGMAFAST^™ 3,3′-Diaminobenzidine tablets	Sigma-Aldrich P9187 SIGMAFAST^™ OPD	Sigma-Aldrich B0274 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
solubility water: 10 mL, clear, yellow	solubility water: 1 tablet/5 mL	solubility water: 0.5 tablet/10 mL, clear, colorless	solubility -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form tablet	form tablet	form tablet	form tablet
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C

Application

SIGMAFAST BCIP^®/NBT has been used as a substrate

for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting[1]
for alkaline phosphatase in the histochemical staining of primary osteoblasts[2]
for alkaline phosphatase staining in bone marrow stromal cells[3]

Preparation Note

Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

저장 등급

11 - Combustible Solids

wgk

WGK 2

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

0000468886

0000442829

0000411613

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence

Davis C, et al.

Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)

Mitogen-activated protein kinase phosphatase 1 regulates bone mass, osteoblast gene expression, and responsiveness to parathyroid hormone

Mahalingam CD, et al.

The Journal of Endocrinology, 211(2), 145-156 (2011)

Amplicon-Based RNA Interference Targeting V2 Gene of Cotton Leaf Curl Kokhran Virus-Burewala Strain Can Provide Resistance in Transgenic Cotton Plants.

Aneela Yasmeen et al.

Molecular biotechnology, 58(12), 807-820 (2016-10-21)

The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct

Intranasal immunization with a single dose of the fusion protein formulated with a combination adjuvant induces long-term protective immunity against respiratory syncytial virus.

R Garg et al.

Human vaccines & immunotherapeutics, 13(12), 2894-2901 (2017-08-22)

Respiratory syncytial virus (RSV) is the most common cause of respiratory tract infections in both children and elderly people. In this study we evaluated the short- and long-term protective efficacy of a single intranasal (IN) immunization with a RSV vaccine

Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells

Teuton JR and Brandt CR

Journal of Virology, 81(8), 3731-3739 (2007)

모든 관련된 서류 보기

문서

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

프로토콜

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

Guide: Expansion & Differentiation of Human Mesenchymal

TrueGel3D^® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

질문

2년 전

Is it ok to store this tablet (sigmafast BCIP/NBT) after being dissolved in water? (in case I couldn't use all 10 mL) If it's ok, which temperature (and other conditions) should I store it?

1 답변
1. Technical Support
  
  2년 전
  
  For best results, it is recommended to use the solution within 1 hour. Long term storage of the solution has not been evaluated. A prepared BCIP/NBT solution is available under product number B6404. This product is prepared in Tris buffer and may be stored at room temperature. See the links below for the product datasheets for each item.
  B5655 Tablets
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/403/473/b5655dat.pdf
  B6404 Premixed Solution
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/116/719/b6404bul.pdf
  
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BCIP^®/NBT