콘텐츠로 건너뛰기
Merck

D8130

5,5′-Dithiobis(2-nitrobenzoic acid)

≥98%, BioReagent, suitable for determination of sulfhydryl groups

동의어(들):

3-Carboxy-4-nitrophenyl disulfide, 6,6′-Dinitro-3,3′-dithiodibenzoic acid, Bis(3-carboxy-4-nitrophenyl) disulfide, DTNB, Ellman’s Reagent

Slide 1 of 9
조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

500 MG

₩56,028

1 G

₩96,121

5 G

₩217,921

10 G

₩394,023

25 G

₩814,944

100 G

₩2,355,511

₩56,028

오늘 배송 가능세부사항

벌크 견적 요청하기

제품정보 (DICE 배송 시 비용 별도)

Linear Formula:
[-SC6H3(NO2)CO2H]2
CAS 번호:
69-78-3
Molecular Weight:
396.35
UNSPSC Code:
12352106
NACRES:
NA.26
PubChem Substance ID:
24894189
EC Number:
200-714-4
Beilstein/REAXYS Number:
1896821
MDL number:
MFCD00007140

주요 문서

모든 문서 보기

다음으로 건너뛰기

기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의

제품 이름

5,5′-Dithiobis(2-nitrobenzoic acid), ≥98%, BioReagent, suitable for determination of sulfhydryl groups

SMILES string

OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

InChI key

KIUMMUBSPKGMOY-UHFFFAOYSA-N

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)

product line

BioReagent

assay

≥98%

form

powder

mp

240-245 °C (dec.) (lit.)

suitability

suitable for determination of sulfhydryl groups

Quality Level

200

유사한 제품을 찾으십니까? 방문 제품 비교 안내

유사한 품목 비교

전체 비교 보기

차이점 표시

1 of 4

이 품목
D218200322123158194
5,5′-Dithiobis(2-nitrobenzoic acid) ≥98%, BioReagent, suitable for determination of sulfhydryl groups

Sigma-Aldrich

D8130

5,5′-Dithiobis(2-nitrobenzoic acid)

5,5′-Dithiobis(2-nitrobenzoic acid) ReagentPlus®, 99%

Sigma-Aldrich

D218200

5,5′-Dithiobis(2-nitrobenzoic acid)

5,5′-Dithio-bis-(2-nitrobenzoic Acid) Sulfhydryl reagent used to characterize reactive SH groups.

Sigma-Aldrich

322123

5,5′-Dithio-bis-(2-nitrobenzoic Acid)

2,2′-Dithiobis(5-nitropyridine) 96%

Sigma-Aldrich

158194

2,2′-Dithiobis(5-nitropyridine)

assay

≥98%

assay

99%

assay

≥98% (titration)

assay

96%

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

form

powder

form

powder

form

solid

form

solid

mp

240-245 °C (dec.) (lit.)

mp

240-245 °C (dec.) (lit.)

mp

-

mp

155-157 °C (lit.)

product line

BioReagent

product line

ReagentPlus®

product line

-

product line

-

suitability

suitable for determination of sulfhydryl groups

suitability

-

suitability

-

suitability

-

Application

Ellman′s reagent maybe used for acetylcholinesterase assay, and to specifically label cysteine residues.[1] It is a sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues.

General description

5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent.[2] It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH.[1] Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3.[3]

저장 등급

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
WXBF7740V
0000413693
0000413693
0000513656
0000513635

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Christian K Riener et al.
Analytical and bioanalytical chemistry, 373(4-5), 266-276 (2002-07-12)
Since its introduction in 1959, Ellman's reagent (5,5'-dithio-bis(2-nitrobenzoic acid)) has been the favorite reagent for spectrophotometric measurement of protein sulfhydryls. Meanwhile however, evidence has accumulated that many protein sulfhydryls give an incomplete reaction with Ellman's reagent, even during prolonged assay
Venkaiah Betapudi et al.
Science translational medicine, 12(527) (2020-01-24)
Chemical warfare nerve agents are organophosphorus chemical compounds that induce cholinergic crisis, leaving little or no time for medical intervention to prevent death. The current chemical treatment regimen may prevent death but does not prevent postexposure complications such as brain
Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination.
P W Riddles et al.
Analytical biochemistry, 94(1), 75-81 (1979-04-01)
Anne Bertling et al.
Arteriosclerosis, thrombosis, and vascular biology, 32(8), 1979-1990 (2012-04-28)
Staphylococcus aureus can induce platelet aggregation. The rapidity and degree of this correlates with the severity of disseminated intravascular coagulation, and depends on platelet peptidoglycans. Surface-located thiol isomerases play an important role in platelet activation. The staphylococcal extracellular adherence protein
Reassessment of Ellman's reagent.
P W Riddles et al.
Methods in enzymology, 91, 49-60 (1983-01-01)
모든 관련된 서류 보기

프로토콜

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

관련 콘텐츠

Reagent Grades for Biochemistry Workflows

Find high-purity biochemicals for every workflow. Explore BioUltra, Molecular Biology, BioReagent, and reagent-grade materials qualified for performance.

Product Information Sheet - D8130

Product Information Sheet

질문

1–4 / 4 질문  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 답변

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      도움이 되었습니까?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 답변

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      도움이 되었습니까?

  3. To dissolve DTNB which alcohol should use?

    1 답변

    1. This product is tested for solubility in Ethanol at 10 mg/mL. Solutions may also be prepared at 5mM in 100 mM sodium or potassium phosphate buffer, pH 7.2, containing 0.1 mM EDTA. Please see the link below to review the entire product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/414/707/d8130pis.pdf

      도움이 되었습니까?

  4. what is the storage temperature for this chemical

    1 답변

    1. This product can be stored at room temperature.

      도움이 되었습니까?

후기

평점 값 없음

활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.