Endotoxin-free products are all typically filtered with a 0.1 micron pore size filter. Sterile filtered products typically have a 0.2 or 0.22 pore size filter.
R8758
RPMI-1640 Medium
With ʟ-glutamine, sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
RPMI-1640 Medium
동의어(들):
Roswell Park Memorial Institute 1640 medium
다음으로 건너뛰기
제품 이름
RPMI-1640 Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Level
무균
sterile-filtered
양식
liquid
기술
cell culture | mammalian: suitable
불순물
endotoxin, tested
성분
NaHCO3: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
L-glutamine: yes
배송 상태
ambient
저장 온도
2-8°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
1 of 4
이 품목 | R0883 | R7638 | R7509 |
|---|---|---|---|
| form liquid | form liquid | form liquid | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| components NaHCO3: yes | components sodium pyruvate: no | components sodium pyruvate: no | components phenol red: no |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
일반 설명
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
애플리케이션
관련 제품
제품 번호
설명
가격
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
E L Davies et al.
Clinical and experimental immunology, 145(1), 183-189 (2006-06-24)
An increasing number of cell types, including peripheral blood mononuclear cells (PBMCs), have been demonstrated to release heat shock proteins (Hsps). In this paper we investigate further the hypothesis that Hsps are danger signals. PBMCs and Jurkat cells released Hsp70
Katarzyna A Podyma-Inoue et al.
Cancer genomics & proteomics, 13(6), 443-452 (2016-11-04)
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Anders Abildgaard et al.
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The gut microbiota has recently emerged as an important regulator of brain physiology and behaviour in animals, and ingestion of certain bacteria (probiotics) therefore appear to be a potential treatment for major depressive disorder (MDD). However, some conceptual and mechanistical
Thaneas Prabakaran et al.
PloS one, 6(9), e25065-e25065 (2011-09-29)
Injury to the glomerular podocyte is a key mechanism in human glomerular disease and podocyte repair is an important therapeutic target. In Fabry disease, podocyte injury is caused by the intracellular accumulation of globotriaosylceramide. This study identifies in the human
Michelle J Nyhan et al.
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Successful treatment of oesophageal cancer is hampered by recurrent drug resistant disease. We have previously demonstrated the importance of apoptosis and autophagy for the recovery of oesophageal cancer cells following drug treatment. When apoptosis (with autophagy) is induced, these cells
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When the medium waas sterile-filtered, which pore size was used?
1 답변-
도움이 되었습니까?
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Good Morning, does RPMI medium R8758 which contains sodium bicarbonate, affect cell suspension culture of megakaryoblastic cell line DAMI? I mean, sodium bicarbonate can significantly modify cell behavior or his effects can be tolerated?
1 답변-
This product has not been validated for use with the megakaryoblastic cell line DAMI. However, literature indicates compatibility with this cell line. The following citation may be of interest: PLoS One. 2020 Apr 7;15(4):e0230507.
도움이 되었습니까?
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I bought this product and realized that it does not contain Hepes. Can I add hepes?
1 답변-
This product utilizes a bicarbonate buffering system. However, there are no anticipated problems with the addition of HEPES. We do offer RPMI formulations containing both sodium bicarbonate and HEPES, such as Products R5886 & R7638.
Please see the RPMI formulation tables at the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdf
도움이 되었습니까?
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Considering that I have to use an RPMI medium containing 4mM of l-Glutamine and 10mM of non-essential amino acids, can I use the product R8748 without adding anything?
1 답변-
The information you are requesting can be supplied by our Technical Service team who can assist you further. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
도움이 되었습니까?
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buenos dias, llevo años usando vuestro producto RPMI 1640, y me gustaría saber la composición del medio para ver si hay algun metal/producto que no puede utilizar con un suplemento que quiero introducir. Muchas gracias
1 답변-
Please see the link below to review the RPMI formulation tables. This product is listed in the second column of page 2:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/203/777/r8758for.pdf도움이 되었습니까?
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
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Does Product R8758, RPMI-1640 Medium, contain glucose?
1 답변-
RPMI contains glucose at 2 g/L. The complete formulation of RPMI and other classical media formulations can be found on the Cell Culture Web Page.
도움이 되었습니까?
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Can Product R8758, RPMI-1640 Medium, be frozen?
1 답변-
We do not recommend freezing cell culture media. A precipitate may form upon thawing. Also, this can cause a loss of amino acids, which may yield low cell growth.
도움이 되었습니까?
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Does Product R8758, RPMI-1640 Medium, contain HEPES?
1 답변-
There is no HEPES in this formulation of RPMI. We do offer RPMI containing HEPES as Product No's. R5886 and R4130. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.
도움이 되었습니까?
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Does Product R8758, RPMI-1640 Medium, contain phenol red?
1 답변-
This media contains phenol red. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.
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