567520-U
Discovery® HS F5 (5 µm) HPLC Columns
L × I.D. 25 cm × 10 mm, HPLC Column
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₩3,712,870
₩3,712,870
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제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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제품 이름
Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
material
stainless steel column
agency
suitable for USP L43
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
12% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 10 mm
surface area
300 m2/g
surface coverage
4 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical particle platform
fully porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
5 μm
pore size
120 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
Quality Level
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이 품목 | 567532-U | 567531-U | 567501-U |
|---|---|---|---|
| matrix active group PFP (pentafluorophenyl) phase | matrix active group PFP (pentafluorophenyl) phase | matrix active group PFP (pentafluorophenyl) phase | matrix active group PFP (pentafluorophenyl) phase |
| particle size 5 μm | particle size 3 μm | particle size 3 μm | particle size 3 μm |
| separation technique reversed phase | separation technique reversed phase | separation technique reversed phase | separation technique reversed phase |
| L × I.D. 25 cm × 10 mm | L × I.D. 15 cm × 4 mm | L × I.D. 10 cm × 4 mm | L × I.D. 3.3 cm × 2.1 mm |
| agency suitable for USP L43 | agency suitable for USP L43 | agency suitable for USP L43 | agency suitable for USP L43 |
| product line Discovery® | product line Discovery® | product line Discovery® | product line Discovery® |
Features and Benefits
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
General description
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Jennie Wang et al.
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An HPLC method for the assay of a DNA topoisomerase inhibitor, LMP776 (NSC 725776), has been developed and validated. The stress testing of LMP776 was carried out in accordance with International Conference on Harmonization (ICH) guidelines Q1A (R2) under acidic
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A "heart-cut" two-dimensional achiral-chiral liquid chromatography triple-quadrupole mass spectrometry method (LC-LC-MS/MS) was developed and coupled to in vivo cerebral microdialysis to evaluate the brain response to the chiral compound (±)-7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine-1,1-dioxide ((±)-1), a potent positive allosteric modulator (PAM) of AMPA receptor.
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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 853(1-2), 265-274 (2007-04-10)
Galantamine, an alkaloid isolated from the bulbs and flowers of Caucasian snowdrop (Galanthus woronowii, Amaryllidaceae) and related species, is employed in human medicine for the treatment of various neuromuscular and neurodegenerative diseases. After the administration, the products of oxidative biotransformation
Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.
Koji Baba et al.
Journal of chromatography. A, 1354, 109-116 (2014-06-21)
Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and
Milan Nobilis et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(32), 3845-3852 (2011-11-22)
New bioanalytical SPE-HPLC-PDA-FL method for the determination of the neuroleptic drug tiapride and its N-desethyl metabolite was developed, validated and applied to xenobiochemical and pharmacokinetic studies in humans and animals. The sample preparation process involved solid-phase extraction of diluted plasma
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