DTT-RO

DTT

crystalline powder, =97% (Ellman′s reagent), M_r 154.3

• Synonym(s): DL-Dithiothreitol, 1,4-dithiothreitol, cleland′s reagent, dithiothreitol, 1,4-, threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
• Linear Formula: HSCH₂CH(OH)CH(OH)CH₂SH
• CAS Number: 3483-12-3
• Molecular Weight: 154.25
• Beilstein: 1719757
• Número MDL: MFCD00004877
• ID de la sustancia en PubChem: 329798930

Properties

• descripción: 1,4-Dithiothreitol
• ensayo: =97% (Ellman′s reagent)
• formulario: crystalline powder
• mol peso: M_r 154.3
• reaction suitability: reagent type: reductant
• envase: pkg of 10 g (10708984001); pkg of 2 g (10197777001); pkg of 25 g (11583786001)
• manufacturer/tradename: Roche
• mp: 41-44 °C (lit.)
• temp. de almacenamiento: 2-8°C
• SMILES string: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• InChI key: VHJLVAABSRFDPM-QWWZWVQMSA-N

Description

Descripción general

1,4-dithiothreitol is commonly known as Cleland′s reagent. It confers protection to thiol groups and reduces disulfide bonds in peptides and proteins. It reduces disulfide bonds to sulfhydryl group and is commonly used in receptor studies, to determine the functional importance of disulfide bonds during receptor occupancy and functionality. It also maintains monothiols completely in the reduced state.

Aplicación

1,4-dithiothreitol is used in:

• Isolation, purification and characterization of proteins and enzymes
• Measurement of enzyme activities (reactivation of enzymes)
• Determination of disulfide groups in proteins and enzymes
• DNA extraction prior to amplification

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Envase

2, 10, 25 g

Especificaciones

Oxidized form: <2.5% (absorbance at 283nm)

Secuencia

Enantiomers
Our DTT-preparation is optically inactive, i.e. it is the D,L-DTT.

Nota de preparación

Storage conditions (working solution): A solution of DTT in Hepes buffer, pH 7.75 is stable for one week at 2 to 8 °C if the container is tightly sealed and the solution is protected from atmospheric oxygen by argon or nitrogen.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Safety Information

• pictogramas: GHS05,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H302 - H315 - H318 - H412
• Consejos de prudencia: P264 - P270 - P273 - P280 - P305 + P351 + P338 + P310 - P501
• Clasificaciones de peligro: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2
• Código de clase de almacenamiento: 11 - Combustible Solids
• WGK: WGK 2