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69892

Sigma-Aldrich

MES monohydrate

BioXtra, ≥99.0% (T)

Sinónimos:
2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid monohydrate
Empirical Formula (Hill Notation):
C6H13NO4S · H2O
Número de CAS:
Peso molecular:
213.25
Beilstein:
6350956
Número de EC:
Número MDL:
eCl@ss:
32129211
ID de la sustancia en PubChem:
NACRES:
NA.25

Nivel de calidad

200

línea de producto

BioXtra

ensayo

≥99.0% (T)

formulario

powder or crystals

intervalo de pH útil

5.5 - 6.7

pKa (25 °C)

6.1

mp

>300 °C (lit.)

solubilidad

H2O: 0.5 M at 20 °C, clear

trazas de anión

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

trazas de catión

Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

application(s)

diagnostic assay manufacturing

SMILES string

O.OS(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S.H2O/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);1H2

InChI key

MIIIXQJBDGSIKL-UHFFFAOYSA-N

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Descripción general

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.

Aplicación

MES Monohydrate has been used:
  • To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay
  • As a buffering agent to stabilize enzymatic solutions
  • As a component of PAGE running buffer

Nota de preparación

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Otras notas

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.

Código de clase de almacenamiento

13 - Non Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

dust mask type N95 (US), Eyeshields, Gloves

Certificado de Análisis

Certificado de origen

Generating heparan sulfate saccharide libraries for glycomics applications.
Powell, AK et al.
Nature Protocols, 5 (5), 821-821 (2010)
In vitro cell wall stress assay for Fusarium oxysporum.
Perez-Nadales, E, et al.
Bio-protocol, 6, e1915-e1915 (2016)
Characterization of soil organic phosphorus in NaOH-EDTA extracts: A comparison of 31P NMR spectroscopy and enzyme addition assays.
Jarosch, KA et al.
Soil Biology and Biochemistry, 91 , 298-309 (2015)
Dawson, R.M.C. et al.
Data for Biochemical Research, 410- 410 (1987)
Good NE et al.
Biochemistry, 5, 467-477 (1966)

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