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Merck

A4544

Ácido L-ascórbico

suitable for cell culture, suitable for plant cell culture, ≥98%

Sinónimos:

Ácido L-treoascórbico, Factor antiescorbútico, Vitamina C

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25 G

MXP 873.00

100 G

MXP 1,705.00

500 G

MXP 4,633.00

MXP 873.00


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Fórmula empírica (notación de Hill):
C6H8O6
Número CAS:
Peso molecular:
176.12
Beilstein/REAXYS Number:
84272
EC Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.21

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Quality Level

assay

≥98%

form

powder

mol wt

Mw 176.12 g/mol

technique(s)

cell culture | mammalian: suitable
cell culture | plant: suitable

color

white to light yellow

pH

1.0-2.5 (25 °C, 176 g/L in water)

mp

190-194 °C (dec.)

solubility

water: soluble 50 mg/mL

SMILES string

OC([C@]([C@@H](O)CO)([H])O1)=C(O)C1=O

InChI

1S/C6H8O6/c7-1-2(8)5-3(9)4(10)6(11)12-5/h2,5,7-10H,1H2/t2-,5+/m0/s1

InChI key

CIWBSHSKHKDKBQ-JLAZNSOCSA-N

Gene Information

human ... SLC23A2(9962)

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Este artículo
A59603303457803
technique(s)

cell culture | mammalian: suitable, cell culture | plant: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

IR spectroscopy: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

assay

≥98%

assay

≥99.0%

assay

99.7-100.5% (oxidimetric)

assay

-

form

powder

form

crystalline

form

solid

form

solid

solubility

water: soluble 50 mg/mL

solubility

H2O: 0.5 M, clear, colorless

solubility

water: soluble 176 g/L at 20 °C

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

mp

190-194 °C (dec.)

mp

190-194 °C (dec.)

mp

190-192 °C ((decomp)) (lit.), 190-194 °C (dec.)

mp

190-194 °C (dec.)

General description

L-ascorbic acid also called vitamin C, is a water soluble vitamin and acts as a reducing agent for various biochemical reactions. Ascorbic acid plays a key role in the production of collagen. Ascorbic acid is essential for neurodevelopment, neurotransmitter synthesis and modulates neuronal synapsis.[1] Dietary inadequacy of vitamin C has negative impact on cognition.[2]

Application

L-ascorbic acid has been used as an ingredient in the basal medium for bovine mammary epithelial cells[3] and oocyte-granulosa cell complexes.[4] It has been used as a supplement in the osteogenic medium for osteogenic differentiation.[5][6]

Biochem/physiol Actions

Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.

Clase de almacenamiento

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Certificados de análisis (COA)

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Visite la Librería de documentos

Vitamin C status and cognitive function: A systematic review
Travica N, et al.
Nutrients, 9(9), 960-960 (2017)
Does vitamin C deficiency affect cognitive development and function?
Hansen SN, et al.
Nutrients, 6(9), 3818-3846 (2014)
The effect of mesenchymal stromal cells on the osseoinduction of impaction grafts
Korda M, et al.
Tissue Engineering: Part A, 16(2), 675-683 (2009)
Mitochondrial dysfunction of in vitro grown rabbit oocytes results in preimplantation embryo arrest after activation
Kanaya H, et al.
Journal of Reproduction and Development, 53(3), 631-637 (2007)
Multipotency and cardiomyogenic potential of human adipose-derived stem cells from epicardium, pericardium, and omentum
Wystrychowski W, et al.
Stem Cell Research & Therapy, 7(1), 84-84 (2016)

Artículos

Importance and uses of ascorbate in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Can we store vitamin C solution (25mM) at -20 degree? In case for how many time it can be stored?

    1 answer
    1. The solution stability of this compound has not been determined, however it is subject to degradation when exposted to oxygen or alkaline pH conditions. As a general rule, avoid repeated freeze/thaw cycles. The publication below indicates that the material is stable when stored frozen in RPMI-1640 at -20°C.
      https://pubmed.ncbi.nlm.nih.gov/1698293/

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