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Merck

CA201

Sigma-Aldrich

cAMP Enzyme Immunoassay Kit

sufficient for 96 assays

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UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 96 assays

Quality Level

technique(s)

ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CAMP(820)

General description

Non-radioactive, competitive immunoassay for the quantitation of cAMP in biological fluids (serum, plasma, and saliva), tissue culture media samples, or in samples containing very low concentrations of cyclic nucleotides. This kit uses a polyclonal antibody to cAMP to competitively bind cAMP or cAMP which has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the additon of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cAMP present in the well.

Application

cAMP Enzyme Immunoassay Kit has been used to quantify the cAMP level.

Other Notes

Please refer to the attached Protocolfor details.

Solo componentes del kit

Referencia del producto
Descripción

  • Acetic Anhydride 1 x 1

  • Assay Buffer 2 1 x 30

  • cAMP-Alkaline Phosphatase Conjugate 1 x 5

  • cAMP Assay Layout Sheet 1 x 1

  • cAMP EIA Antibody Rabbit Anti-cAMP 1 x 5

  • 5 Cycle Logit-Log Paper 1 x 1

  • Cyclic AMP Standard 1 x 0.5

  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea

  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20

  • Plate Sealer 1 ea

  • Stop Solution 1 x 5

  • Triethylamine 1 x 2

  • Wash Buffer Concentrate 1 x 30

Ver todo (13)

signalword

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1A - STOT SE 3

target_organs

Respiratory system

supp_hazards

wgk_germany

WGK 3

flash_point_f

5.0 °F

flash_point_c

-15 °C


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