MP0025

Sigma-Aldrich

Venor GeM Mycoplasma Detection Kit, PCR-based

NACRES:
NA.75

Quality Level

usage

1 kit sufficient for 25 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

application(s)

PCR: suitable
detection: suitable
microbiology: suitable

storage temp.

2-8°C

Categorías relacionadas

General description

The Venor GeM Mycoplasma Detection Kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. The primer set is specific to the highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. This allows for detection of all Mycoplasma, Acholeplasma and Ureaplasma species tested so far, which are usually encountered as contaminants in cell cultures. Eukaryotic and bacterial DNA are not amplified by this kit.

Application

The PCR-based Venor GeM Mycoplasma Detection Kit employs PCR technology for rapid and reliable detection of mycoplasma DNA in cell cultures and virus stocks.

Components

Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase

Storage and Stability

The kit components are stable during shipping at ambient temperature. Upon receipt, store at 2–8 °C. After rehydration of the primer/nucleotide mix, the positive control, and the internal control, store below –20 °C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, primer/nucleotide mix and controls should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the label.

Legal Information

Venor is a trademark of Minerva Biolabs GmbH

Solo componentes del kit

Referencia del producto
Descripción

  • Positive Control

  • Negative Control

  • PCR 10X Reaction Buffer 500 mL/vial

  • Primer/Nucleotide Mix 1 mL/vial

RIDADR

NONH for all modes of transport

Certificado de Análisis

Certificado de origen

  1. Product MP0025, VenorGeM® Mycoplasma Kit, does not contain TAQ.  What  DNA polymerase should be used?

    Any DNA polymerase with a 3’-5’ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that can be used include Taq, Tfl, and Tth. The kit has been optimized for use with Product No. D9307.

  2. When using Product MP0025, VenorGeM® Mycoplasma Kit, there is inhibition in the sample (no internal control band).  Can the sample be diluted and the assay repeated?

    Sigma Aldrich does not recommend diluting the sample and re-assay as this can result in false negatives (sample diluted below the detection limits of the assay).  Sigma Aldrich recommends that PCR inhibitors be removed using a DNA extraction with  commercially available kits. (Product Codes G1N10, G1N70, NA2000).

  3. Can a cell lysate be used in the VenorGeM® Mycoplasma Kit, Product MP0025, assay?

    A cell lysate can contain PCR inhibitors.  Sigma Aldrich recommends that PCR inhibitors be removed using a DNA extraction with  commercially available kits. (Product Codes G1N10, G1N70, NA2000).

  4. What is the difference between Product No. MP0025 (VenorGem Mycoplasma Detection Kit) and Product No. MP0035 (Lookout Mycoplasma Detection Kit)?

    The assay kits have a similar range of species detected (see product insert for listing).  For Product No. MP0025, the components are supplied as individual reagents. For Product No. MP0035, the components are supplied in reaction tubes, already mixed and ready for addition of sample and Taq. The internal control for Product No. MP0035 is at 481 bp rather than 191 bp as found in Product No. MP0025.

  5. The sample required for VenorGeM® Mycoplasma Kit, Product MP0025, is a cell culture supernatant.  Is this from adherent or suspension cells?

    The mycoplasma assay kit can be run on supernatants from either adherent or suspension cells. The centrifugation step during sample preparation will remove any cellular debris to limit PCR inhibitors.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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