P9155

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 30,000-70,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Sinónimos: PDL HBr
• Número de CAS: 25988-63-0
• Número MDL: MFCD00237230
• Código UNSPSC: 12352202
• eCl@ss: 32160406
• ID de la sustancia en PubChem: 24899014
• NACRES: NA.75

Propiedades

• Nombre del producto: Poli-L-lisina hydrobromide, mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• origen biológico: synthetic (organic)
• Nivel de calidad: 200
• esterilidad: γ-irradiated
• Línea del producto: BioXtra
• Formulario: lyophilized powder
• mol peso: 30,000-70,000
• envase: pkg of 5 mg
• técnicas: cell culture | mammalian: suitable
• cobertura de la superficie: 4 μg/cm²
• solubilidad: H₂O: soluble 50 mg/mL, clear, colorless
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C
• cadena SMILES: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• Clave InChI: WBSCNDJQPKSPII-KKUMJFAQSA-N

Descripción

Descripción general

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties. Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.[1]

Aplicación

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.[1]
Poly-L-lysine hydrobromide has been used to adhere the dissociated cells of cortical tissues into the culture plates.[2] It has also been used to treat slides, to fix samples on it for immunohistochemical analysis.[3]

Acciones bioquímicas o fisiológicas

Poly-L-lysine is employed as a coating substance to facilitate improved cell attachment to culture surfaces.[4] Research indicates that plates coated with poly-L-lysine (PLL) exhibit an accelerated cell growth rate.[5] When mesenchymal stem cells (MSCs) are cultured on PLL-coated substrates, it helps preserve their stemness properties and prevents senescence. PLL-coated plates promote enhanced MSC growth and up-regulation of genes associated with cell adhesion, differentiation, proliferation, and signaling. Furthermore, porous PLL-coated surfaces have been shown to enhance cell proliferation and support the osteogenic differentiation of MSCs.[6]

Componentes

La poli-L-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-L-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura beta porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.

Precaución

Las disoluciones estériles son estables durante un máximo de 2 años cuando se conservan entre 2 y 8°C. Deben guardarse desecadas a -20°C.

Nota de análisis

Molecular weight based on viscosity. Also assayed by MALLS.

Otras notas

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)