Merck
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P9155

Sigma-Aldrich

Poly-L-lysine hydrobromide

mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Synonym(s):
PDL HBr
CAS Number:
Número MDL:
eCl@ss:
32160406
ID de la sustancia en PubChem:
NACRES:
NA.75

Nivel de calidad

sterility

γ-irradiated

Línea del producto

BioXtra

formulario

lyophilized powder

mol peso

30,000-70,000

envase

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

solubilidad

H2O: soluble 50 mg/mL, clear, colorless

enviado en

ambient

temp. de almacenamiento

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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Descripción general

Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.

Aplicación

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
Poly-L-lysine hydrobromide has been used to adhere the dissociated cells of cortical tissues into the culture plates. It has also been used to treat slides, to fix samples on it for immunohistochemical analysis.

Envase

5 mg

Componentes

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Precaución

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Nota de análisis

Molecular weight based on viscosity. Also assayed by MALLS.

Otras notas

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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