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TP0100

Sigma-Aldrich

Total Protein Kit, Micro

Synonym(s):
Bradford

Nivel de calidad

100

descripción

wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L

parámetro

18-26 °C temperature
50 μL sample volume

temp. de almacenamiento

2-8°C

Descripción general

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Aplicación

Total protein kit, micro has been used to determine:
  • total protein in the bacterial homogenate by Bradford method
  • total protein content by the Bradford microassay from the tissue extracts
  • serum total protein
  • soluble protein content by the Bradford method from leaves

Acciones bioquímicas o fisiológicas

Total protein kit, micro can be used to determine the concentration of proteins in solution.

Idoneidad

Suitable for protein determination for samples of up to 0.5 mg/ml.

Principio

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.

Método de análisis

Colorimetric, Endpoint

Kit Components Also Available Separately

Product No.
Description
SDS

  • Protein Standard 5 mL

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1

Código de clase de almacenamiento

3 - Flammable liquids

WGK

WGK 3

Punto de inflamabilidad F

132.8 °F

Punto de inflamabilidad C

56 °C

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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