Merck
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TP0200

Sigma-Aldrich

Total Protein Kit, Micro-Lowry, Onishi & Barr Modification

sufficient for ~50 manual assays

NACRES:
NA.32

Nivel de calidad

200

descripción

wavelength 500-750nm

uso

sufficient for ~50 manual assays

protein detection level measuring range

0-1 mg/mL

parámetro

18-26 °C temperature
200 μL sample volume

temp. de almacenamiento

2-8°C

Descripción general

The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.

Aplicación

Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
  • in the dialyzates/extracts of gliadins
  • in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoaSalvia hispanica L.)
  • in the stored samples of rat liver microsomal fractions

Principio

Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.

Kit Components Also Available Separately

Product No.
Description
SDS

  • B3934Biuret reagent, protein detection level 150-1,000 μg/mL 110 mL

  • Folin & Ciocalteu’s phenol reagent 5 mL

  • Protein standard 5 mL

pictogramas

Corrosion

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1

Código de clase de almacenamiento

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

Quotes and Ordering

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