03-0155-00

SMC^® Human IL-6 High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-6 in human serum and plasma

• Synonym(s): B-cell stimulatory factor 2 immunoassay kit, IL 6 immunoassay kit, IL-6 Single Molecule Counting Kit, IL-6 ultra-sensitive immunoassay
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• description: 0.01-0.15 pg/mL, refer to CoA for lot-specific LLOQ
• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• manufacturer/tradename: SMC^®
• assay range: sensitivity: 0.08 pg/mL; (lower limit of quantification); inter-assay cv: <20%; intra-assay cv: <15%; (Sensitivity: LLOQ 88 fg/mL* (SMCxPRO^® Instrument); Standard Curve Range: 10-50,000 fg/mL; Linearity: Serum: 115% Plasma: 112%; Recovery: Serum: 112% Plasma: 100%; *Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.)
• technique(s): single molecule counting: suitable
• input: plasma (K2 EDTA); serum
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• shipped in: wet ice

Description

General description

Interleukin 6 (IL-6) is secreted by T cells and macrophages and acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Some of its functions include the stimulation of the immune response during infection, induction of fever in autoimmune, infectious and non-infectious diseases, the stimulation of acute phase reactions, differentiation of T cells, proliferation and differentiation of B-cells, the development of blood cells as well as the activation of osteoclasts and osteoporosis and the secretion of vascular endothelium growth factor (VEFG) to increase blood vessel growth and vascular permeability in inflammation. It is important for a short-term defense against infection, injury and inflammation however dysregulation of IL-6 results in disease.

IL-6 is important in the study of diseases such as diabetes, lupus, multiple myeloma, rheumatoid arthritis and some cancers such as prostate and pancreatic cancer. Conversely, deficiency of IL-6 can have an effect on immune activation and IgA antibodies while overexpression of IL-6 can result in an immunological imbalance leading to an autoimmune pathology.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Biochem/physiol Actions

Although IL-6 was detectable in non-human primate (rhesus macaque and cynomolgus monkey) samples, the assay has not been verified for these species.

Features and Benefits

• Ultra-Low Detection Limits: Enables femtogram-level detection of IL-6, far surpassing traditional ELISA sensitivity.
• Expanded Dynamic Range: Reduces background noise, improving assay performance.
• Targeted Research Applications: Ideal for sudies in inflammation, autoimmune diseases, and biomarker discovery.
• Streamlined Lab Operations: Automation-ready reagents help optimize workflow and reduce manual handling.
• Robust Performance: Supports preclinical and translational research with dependable results, enhancing quality of scientific investigations.
• Advanced Technology: Powered by SMC^®Technology for enhanced sensitivity.

Packaging

96 well assay

Analysis Note

Inflammation & Immunology Inflammatory Disease Immune Response Autoimmune Disease

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H319,H360FD,H411
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Repr. 1B
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects