CCYTOMAG-90K

MILLIPLEX^® Canine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

Name: MILLIPLEX<SUP>®</SUP> Canine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
Brand: MM

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

Synonym(s):

canine interleukin immunoassay panel, luminex canine cytokine chemokine growth factor multiplex assay, luminex canine pro inflammatory anti inflammatory cytokine panel, millipore canine inflammation multiplex kit

Slide 1 of 2

About This Item

UNSPSC Code:

12161503

eCl@ss:

32161000

NACRES:

NA.47

To order a Milliplex® kit, please search for your analyte of interest.

Design And Price Your Kit

Key Documents

View All Documentation

Quality Level

200

species reactivity

canine

manufacturer/tradename

Milliplex^®

assay range

accuracy: 91-109%
sensitivity: 13.6 pg/mL
(IFN-γ)
sensitivity: 21.0 pg/mL
(MCP-1)
sensitivity: 21.7 pg/mL
(IL-8)
sensitivity: 3.2 pg/mL
(IP-10)
sensitivity: 3.5 pg/mL
(IL-2)
sensitivity: 3.7 pg/mL
(IL-6)
sensitivity: 5.3 pg/mL
(KC-like)
sensitivity: 5.8 pg/mL
(IL-18)
sensitivity: 6.1 pg/mL
(TNFα)
sensitivity: 7.5 pg/mL
(IL-7)
sensitivity: 8.5 pg/mL
(IL-10)
sensitivity: 9.0 pg/mL
(IL-15)
sensitivity: 9.2 pg/mL
(GM-CSF)
standard curve range: 12.2-50,000 pg/mL
(All analytes)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex^® xMAP^® technology)

shipped in

wet ice

Related Categories

Immunoassay Platform Solutions

MILLIPLEX^® Multiplex Assays for Luminex^® Instruments

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	CCYTMG-90K-PX13	FCYTMAG-20K-PMX	HCYP3MAG-63K
Millipore CCYTOMAG-90K MILLIPLEX^® Canine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay	Millipore CCYTMG-90K-PX13 MILLIPLEX^® Canine Cytokine/Chemokine Magnetic Bead Panel - Premixed 13 Plex - Immunology Multiplex Assay	Millipore FCYTMAG-20K-PMX MILLIPLEX^® Feline Cytokine/Chemokine Magnetic Bead Panel Premixed 19 Plex, FCYTOMAG-20K-PMX - Immunology Multiplex Assay	Millipore HCYP3MAG-63K MILLIPLEX^® Human Cytokine/Chemokine Magnetic Bead Panel III - Immunology Multiplex Assay
species reactivity canine	species reactivity canine	species reactivity feline	species reactivity human
assay range accuracy: 91-109%, sensitivity: 21.0 pg/mL (MCP-1), sensitivity: 3.2 pg/mL (IP-10), sensitivity: 3.7 pg/mL (IL-6), sensitivity: 5.8 pg/mL (IL-18), sensitivity: 7.5 pg/mL (IL-7), sensitivity: 9.0 pg/mL (IL-15), standard curve range: 12.2-50,000 pg/mL (All analytes), sensitivity: 21.7 pg/mL (IL-8), sensitivity: 5.3 pg/mL (KC-like), sensitivity: 8.5 pg/mL (IL-10), sensitivity: 13.6 pg/mL (IFN-γ), sensitivity: 6.1 pg/mL (TNFα), sensitivity: 3.5 pg/mL (IL-2), sensitivity: 9.2 pg/mL (GM-CSF)	assay range accuracy: 91-109%, sensitivity: 13.6 pg/mL (IFN-γ), sensitivity: 21.0 pg/mL (MCP-1), sensitivity: 21.7 pg/mL (IL-8), sensitivity: 3.2 pg/mL (IP-10), sensitivity: 3.5 pg/mL (IL-2), sensitivity: 3.7 pg/mL (IL-6), sensitivity: 5.3 pg/mL (KC-like), sensitivity: 5.8 pg/mL (IL-18), sensitivity: 6.1 pg/mL (TNFα), sensitivity: 7.5 pg/mL (IL-7), sensitivity: 8.5 pg/mL (IL-10), sensitivity: 9.0 pg/mL (IL-15), sensitivity: 9.2 pg/mL (GM-CSF), standard curve range: 12.2-50,000 pg/mL (All analytes)	assay range accuracy: 96-114%, standard curve range: 13.7-10,000 pg/mL (sFas, IL-2, GM-CSF, IL-8, IL-12 (p40), IL-13), standard curve range: 137.2-100,000 pg/mL (IL-18, MCP-1), standard curve range: 2.7-2,000 pg/mL (KC, RANTES), standard curve range: 274.3-200,000 pg/mL (PDGF-BB), standard curve range: 34.3-25,000 pg/mL (IL-1β), standard curve range: 6.9-5,000 pg/mL (Flt3 Ligand), standard curve range: 68.6-50,000 pg/mL (IFNγ, IL-4, IL-6, SCF, SDF-1)	assay range accuracy: 92-106%, standard curve range: 2.0-200,000 pg/mL
manufacturer/tradename Milliplex^®	manufacturer/tradename Milliplex^®	manufacturer/tradename Milliplex^®	manufacturer/tradename Milliplex^®
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
technique(s) multiplexing: suitable	technique(s) multiplexing: suitable	technique(s) multiplexing: suitable	technique(s) multiplexing: suitable
detection method fluorometric (Luminex xMAP)	detection method fluorometric (Luminex xMAP)	detection method fluorometric (Luminex xMAP)	detection method fluorometric (Luminex xMAP)

General description

In addition to studying inflammation for dog nutrition and disease research, canine animal models are important for human biomedical research and drug development. Cytokine and chemokines are molecular mediators of immune cell response in inflammation and immunity. Quantification of cytokines and chemokines is essential to understanding the immune system, as well as for researching disease states such as autoimmune, allergic reactions, sepsis, and cancer.

The MILLIPLEX^® Canine Cytokine Panel is 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in canine serum, plasma, tissue/cell lysate and culture supernatant samples: GM-CSF, IFNγ, KC-like, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, MCP-1, and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Application

Analytes: GM-CSF, IFNγ, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, KC-like, MCP-1, and TNF-α.
Recommended Sample Type: Canine serum, plasma, cell culture supernatant and tissue/cell lysates
Recommended Sample Dilution: 25 μL of a 1:3 dilution of plasma or serum, or 25 μL cell culture supernatant
Assay Run Time: Overnight at 4°C or 2 hours at room temperature (20-25° C)
Research Category: Inflammation & Immunology

Biochem/physiol Actions

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

There was no or negligible cross-reactivity among different analytes within the panel.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Preparation Note

Recommended storage for kit components is 2 - 8°C

Other Notes

Please contact Technical Service for linearity of dilution.

Replaces: CCYTO-90K

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

GHS08,GHS05,GHS07,GHS09

Signal Word

Danger

Hazard Statements

H302 + H312 + H332,H317,H318,H373,H411

Precautionary Statements

P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Association of Canine Osteosarcoma and Monocyte Phenotype and Chemotactic Function.

J L Tuohy et al.

Journal of veterinary internal medicine, 30(4), 1167-1178 (2016-06-25)

Monocytes/macrophages are likely key cells in immune modulation in dogs with osteosarcoma (OSA). Increased peripheral monocyte counts are negatively correlated with shorter disease-free intervals in dogs with OSA. Understanding the monocyte/macrophage's modulatory role in dogs with OSA can direct further

Effect of immunosuppressive drugs on cytokine production in canine whole blood stimulated with lipopolysaccharide or a combination of ionomycin and phorbol 12-myristate 13-acetate.

Julien R S Dandrieux et al.

Veterinary medicine and science, 5(2), 199-205 (2019-01-22)

A pharmacodynamic assay has been previously developed to monitor ciclosporin treatment in dogs by assessing inhibition of cytokine transcription after whole blood stimulation with 12-myristate 13-1 acetate and ionomycin (PMA/I). In this study, whole blood stimulation with either PMA/I or

Phase I trial of convection-enhanced delivery of IL13RA2 and EPHA2 receptor targeted cytotoxins in dogs with spontaneous intracranial gliomas.

John H Rossmeisl et al.

Neuro-oncology, 23(3), 422-434 (2020-08-20)

The interleukin-13 receptor alpha 2 (IL13RA2) and ephrin type A receptor 2 (EPHA2) are attractive therapeutic targets, being expressed in ~90% of canine and human gliomas, and absent in normal brain. Clinical trials using an earlier generation IL-13 based cytotoxin

Immunologic Effects of Stereotactic Body Radiotherapy in Dogs with Spontaneous Tumors and the Impact of Intratumoral OX40/TLR Agonist Immunotherapy.

Mary-Keara Boss et al.

International journal of molecular sciences, 23(2) (2022-01-22)

Stereotactic body radiotherapy (SBRT) is known to induce important immunologic changes within the tumor microenvironment (TME). However, little is known regarding the early immune responses within the TME in the first few weeks following SBRT. Therefore, we used the canine

High Mobility Group Box-1 and Pro-inflammatory Cytokines Are Increased in Dogs After Trauma but Do Not Predict Survival.

Robert Goggs et al.

Frontiers in veterinary science, 5, 179-179 (2018-08-15)

Trauma is common in dogs and causes significant morbidity and mortality, but it remains challenging to predict the prognosis of dogs with traumatic injuries. This study aimed to quantify plasma high-mobility group box-1 (HMGB-1) and cytokine concentrations in dogs with

View All Related Papers

Questions

Anonymous

2 years ago

Are fecal samples compatible with Milliplex Immunoassays?

1 answer
1. Technical Support
  
  2 years ago
  
  Fecal contents are collected and weighed, then resuspended in PBS + 5% gelatin supplemented with a complete inhibitor cocktail. The samples are extracted with vortexing and prepared in a concentrated form to facilitate the detection of low levels of cytokines.
  The insoluble fraction is separated by centrifugation at greater than 10,000xg for 10 minutes at 4°C. The resulting supernatants can be assayed immediately or frozen at -80°C.
  It may be necessary to test an optimal dilution, using the extraction buffer as the assay matrix for standards and blanks.
  
  Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

MILLIPLEX® Canine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay