Product 03978 is not a GMP quality product. No testing is performed during Quality Control testing that measures lot to lot variability. The testing includes tests for Appearance, Thin Layer Chromatography, Density, Wavelength 1 at 656-666nm, Extinction Coefficient for Wavelength 1 and the solvent used to measure the wavelength and extinction coefficient. The information about a 1.5% concentration of dye is from the production documents used to manufacture the dye solution. The actual dye concentration is not measured.
03978
Methylene Blue solution
concentrate according to Ehrlich, concentrated, aqueous solution
Synonym(s):
Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue
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About This Item
Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
Molecular Weight:
319.85
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
3641570
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Product Name
Methylene Blue solution, concentrate according to Ehrlich, concentrated, aqueous solution
SMILES string
[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
InChI key
CXKWCBBOMKCUKX-UHFFFAOYSA-M
InChI
1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
form
liquid
quality
concentrated, aqueous solution
shelf life
~3 yr
concentration
1.5% (1.5 g/ 100 mL)
density
1.0 g/mL at 20 °C
εmax
9.4 at 656-666 nm in water
suitability
suitable for microscopy
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
Quality Level
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Related Categories
1 of 4
This Item | 319112 | 457250 | 1808 |
|---|---|---|---|
| grade for microscopy | grade - | grade - | grade - |
| concentration 1.5% (1.5 g/ 100 mL) | concentration 0.05 wt. % in H2O | concentration - | concentration 1.4 % (w/v) in 95% ethanol |
| form liquid | form liquid | form solid | form solution |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| shelf life ~3 yr | shelf life - | shelf life - | shelf life - |
| storage temp. room temp | storage temp. room temp | storage temp. 10-30°C | storage temp. 15-25°C |
Application
Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability.[1] It has also been used to stain sections to determine the presence of mycobacteria.[2][3]
Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.
General description
Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Concerning the stated concentration of the product 03978 (Methylene blue solution, 1.5%), what is the uncertainty and lot-to-lot variability?
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