After the initial thaw, the serum can be aliquoted in to single use vials and stored at -20°C. For more information, please refer to the following page:
https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-culture-media-preparation/storing-and-thawing-serum
H4522
Human Serum
from human male AB plasma, USA origin, sterile-filtered
Synonym(s):
human plasma, human sera, human serum
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About This Item
Biological source:
human male plasma (Type AB)
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
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biological source
human male plasma (Type AB)
Quality Level
sterility
sterile-filtered
composition
hemoglobin, ≤30 mg/dL
origin
USA origin
technique(s)
cell culture | mammalian: suitable
cell culture | stem cell: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
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1 of 4
This Item | H3667 | P2918 | H6914 |
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable |
| biological source human male plasma (Type AB) | biological source human male plasma (Type AB) | biological source human | biological source human |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| composition hemoglobin, ≤30 mg/dL | composition hemoglobin, ≤20 mg/dL | composition - | composition Hemoglobin, ≤25 mg/dL |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities (mycoplasma tested, virus tested), ≤10 EU/mL endotoxin | impurities ≤10 EU/mL Endotoxin |
Application
Human AB serum is used in tissue engineering, transplantation and cell therapy applications for the expansion of mesenchymal stem cells (MSC) from adipose tissue or mesenchymal stromal cells from human bone marrow; for standardized limbal epithelial stem cell graft generation and transplantation; for ex vivo expansion of NK cells from peripheral blood in Hematopoeitic Stem Cell Expansion Medium; and for upgrading pretransplant human islet culture technology.
Human Serum has been used: as a component of phosphate-buffered saline with Tween® detergent (PBST) in bridging enzyme-linked immunosorbent assay (ELISA), as a mock biological sample for assessing the performance of paper-based ELISA assay with complex biological samples, as a diluent to create a dilution series of the baseline sample for the generation of standard curve for NISTmAb spiked into serum, as well as for multiple myeloma (MM) patient sample MM2232
Biochem/physiol Actions
Human AB serum is typically obtained by allowing whole blood to clot naturally, which is known as Off-The-Clot (OTC) serum. Human serum obtained from clotted whole blood is preferred as it usually contains higher levels and a more diverse range of growth factors for optimal cell growth. Human serum from blood-type AB donors lacks antibodies specific for both A and B blood-type antigens. This makes it a universal donor serum that is suitable for various cell culture types including human stem cell lines.
Packaging
Packaging may vary for different batches due to short supply of the bottles used to pack this product. The presence or lack of shrink wrap around the cap of the bottle doesn’t impact product integrity/sterility of the product.
Analysis Note
Endotoxin tested
Other Notes
It is not unusual for our human serum products to be cloudy. This product is from pooled serum and cloudiness indicates that lipids are present in the serum. No clear, lipid-free human serum products are available.
Legal Information
TWEEN is a registered trademark of Croda International PLC
Disclaimer
Each donor is tested for and found non-reactive for Hepatitis B & C and non-reactive for Human Immunodeficiency Virus (HIV) antibody by ELISA. Nevertheless, products of human origin should be considered potentially infectious and handled accordingly.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.
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Once the product has been thawed, how should it be stored? How many times can be freezed and thawed?
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Is this Human Serum stock in PBS or any specific buffer?
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This product does not contain phosphate-buffered saline or any specific buffer.
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What is the concentration of Mg2+ and Ca2+ in this serum (H4522)?
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The magnesium levels are not determined for this serum product. The calcium levels are not tested on a lot-to-lot basis. However, historical information indicates calcium concentrations tend to range from 6.5 - 10 mg/dL.
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What is the antibodies concentration in Product H4522, human serum?
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The immunoglobulin concentration of this product is not measured. This is a pooled product from adult AB negative males. Based on information published in the Geigy Scientific Tables, the average immunoglobulin levels for adult males, ages 20 – 70, are as follows:
IgG
10.69 – 12.04 g./L
IgA
1.32 – 2.34 g/L
IgM (not age dependent)
0.77 g/LHelpful?
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Il prodotto è stato testato per negatività a HSV?
1 answer-
Each donor is tested for and found non-reactive to Hepatitis B & C and non-reactive to Human Immunodeficiency Virus (HIV) antibody by ELISA. HSV is not on the list of agents that are tested. Nevertheless, products of human origin should be considered potentially infectious and handled accordingly. See the link below to review a sample or lot specific Certificate of Analysis for this product: https://www.sigmaaldrich.com/product/sigma/i4506#product-documentation
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If this is "serum" derived from "plasma" the plasma would have contained anti-coagulant. To turn the plasma into serum it has to be clotted. Has this product been clotted to remove the fibrin? Or is it still in fact plasma?
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The product has been clotted. Blood is collected and treated with citrate (anticoagulant) which inhibits clotting initially. The blood is then centrifuged. When the spin is complete, layers will appear which allow a differentiation between the cells and the plasma. The plasma is removed and calcium is added to activate the clotting cascade. The preparation is then centrifuged again and the result is a large clot which is pelleted to the bottom of the tube with a serum layer on top.
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Are Human Serum products H4522 and H6914 suitable for complement assays? Is there testing available for complement levels or activity?
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Human Serum products H4522 and H6914 may not be suitable for complement assays when stored at -20°C due to potential degradation of complement activity. For complement assays, alternatives like S1764 (Complement sera human) and C9473 (Complement Serum Standard human) are recommended. It is advised to review the Certificates of Analysis (CoAs) for these products to ensure they meet the specific requirements of your complement assay
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Which human serum is recommend for a nucleic acid stability assay, specifically for incubating siRNA in serum to represent bloodstream kinetics?
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The use of human serum for a nucleic acid stability assay has not been performed as it is not part of our lab's product characterization focus. However, the citation titled "Improving gene silencing of siRNAs via tricyclo-DNA modification, PMID: 21687522" suggest that product H4522 may be suitable for this application. It is recommended to refer to the publication for additional insights.
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What are the differences between serum and plasma-derived serum?
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Whole blood serum is obtained by allowing natural clotting followed by filtration,lacking fibrinogen. Plasma-derived serum is obtained from non-clotted whole blood through centrifugation.
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What Biosafety Level would be suitable for human serum such as H4522, H5667, and H6914?
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Human origin products from normal donors should be used at Biosafety Level 2 (BSL-2), which is suitable for handling moderate-risk agents causing human disease. Thus, treat these products as potentially infectious and handle accordingly.
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