This product contains four phosphatase inhibitors. For use, one mL of cocktail solution is used to prepare 100 mL of supernatant that contains a maximum of 500 mg of protein. This results in a 1:100 dilution.
P5726
Phosphatase Inhibitor Cocktail 2
aqueous solution (dark coloration may develop upon storage, which does not affect the activity), for the inhibition of tyrosine protein phosphatases, acid and alkaline phosphatases
Synonym(s):
Protease inhibitor
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.54
Form:
aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
Solubility:
water: soluble
Storage temp.:
2-8°C
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Product Name
Phosphatase Inhibitor Cocktail 2, aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
form
aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
solubility
water: soluble
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Related Categories
1 of 4
This Item | PPC1010 | P8465 | 539132 |
|---|---|---|---|
| form aqueous solution (dark coloration may develop upon storage, which does not affect the activity) | form DMSO solution | form lyophilized powder | form lyophilized |
| Quality Level 300 | Quality Level 200 | Quality Level 300 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in wet ice |
Application
The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for tyrosine protein phosphatases, acid and alkaline phosphatases. It has been tested on cell extracts from animal tissues including bovine liver and human placenta, and cytosolic extract of rabbit muscle.
Biochem/physiol Actions
This mixture contains individual components with specific inhibitory properties. Sodium orthovanadate inhibits a number of ATPases, protein tyrosine phosphatases, and other phosphate-transferring enzymes. Sodium molybdate inhibits acid and phosphoprotein phosphatases. Sodium tartrate inhibits acid phosphatases. Imidazole inhibits alkaline phosphatases.
Disclaimer
As supplied, this product is stable for two years at the recommended 2-8°C storage temperature.
Features and Benefits
This compound is a featured product for Kinase Phosphatase Biology research. Click here to discover more featured Kinase Phosphatase Biology products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
General description
Phosphatase Inhibitor Cocktail 2 is a mixture of components designed to inhibit enzymes that modify proteins present in cell extracts, leading to improved yields of intact proteins. It has been optimized and tested for use on various animal tissues and cytosolic extracts.
Preparation Note
This cocktail is supplied as a clear aqueous solution. It has been sterile filtered through a 0.2 μm membrane in bottles that have been aseptically filled. One mL will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, bovine liver, rabbit muscle, A431, or Jurkat cell extracts at a protein concentration of approximately 5 mg/mL.
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Description
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Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
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Related Content
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What is the concentration of the inhibitor solution? 100x?
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Does this contain any metal chelators?
1 answer-
This phosphatase inhibitor cocktail does not contain a chelator. A phosphatase inhibitor cocktail that includes EDTA is not offered at this time. Protease inhibitor cocktails with chelators are offered.
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