P7119

PGO Enzyme Preparation

Name: PGO Enzyme Preparation
Brand: SIGMA

1 G capsules

Synonym(s):

Peroxidase - Glucose oxidase preparation

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About This Item

NACRES:

NA.54

UNSPSC Code:

12352204

Key Documents

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Product Name

PGO Enzyme Preparation, 1 G capsules

form

powder

solubility

H₂O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)

storage temp.

2-8°C

Quality Level

200

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This Item	345386	G6125	P8125
Sigma-Aldrich P7119 PGO Enzyme Preparation	Sigma-Aldrich 345386 Glucose Oxidase, Aspergillus niger, Recombinant	Sigma-Aldrich G6125 Glucose Oxidase from Aspergillus niger	Sigma-Aldrich P8125 Peroxidase from horseradish
form powder	form lyophilized solid	form powder	form essentially salt-free, lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C
solubility H₂O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)	solubility 0.1 M phosphate buffer, pH 7.0: 5 mg/mL	solubility -	solubility 0.1 M phosphate buffer: soluble (pH 6.0), H₂O: soluble
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 200

Application

PGO Enzymes preparation has been used:

in diet sampling and chemical analyses[1][2]
to determine plasma and follicular fluid glucose levels[3]
to measure glucose content in fractions of retrograded debranched rice starch[4]

Tested and found suitable for use in the quantitation of glucose.

Biochem/physiol Actions

PGO Enzymes are used for the quantitative, enzymatic determination of glucose in aqueous solutions such as serum. The reactions are normally monitored at 425-475 nm utilizing o-dianisidine as a colorimetric substrate. Glucose oxidase methods eliminate the effects of interfering substances. Contents of one capsule includes 100 units peroxidase and 500 units glucose oxidase and buffer salts.

General description

Each capsule contains 500 units of glucose oxidase (Aspergilus niger), 100 purpurogallin units of peroxidase (horseradish), and buffer salts. The reaction produces oxidized o-Dianisidine, which is brown. The intensity of the brown color measured at 425-475 nm is proportional to the original glucose concentration.

Preparation Note

The PGO enzymes solution is prepared by adding the contents of 1 capsule of PGO enzymes to 100 mL of water in an amber bottle. Bottle should be inverted several times with gentle shaking to dissolve. PGO Enzymes solution should be stored at 2-8 °C. The solution is stable for up to 1 month unless turbidity develops, and for at least 6 months at –20 °C.

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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