RDD002

HEPES

≥99.5%, crystalline powder, anhydrous, Redi-Dri^™

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• NACRES: NA.25
• PubChem Substance ID: 329824215
• UNSPSC Code: 12161700
• EC Number: 230-907-9
• MDL number: MFCD00006158
• Beilstein/REAXYS Number: 883043

Properties

• Product Name: HEPES, anhydrous, free-flowing, Redi-Dri^™, ≥99.5%
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• grade: anhydrous
• product line: Redi-Dri^™
• assay: ≥99.5%
• form: crystalline powder
• quality: free-flowing
• pH: 5.0-6.5 (25 °C, 238 g/L)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: water: 500 mg/mL, clear, colorless
• Quality Level: 100

Description

Application

HEPES has been used:

• In the novel vesicle-budding protocol as a component in stock solution and buffer solutions[1]
• As a component in intracellular solution for the preparation of brain-slice recording[2]
• As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification[3]
• To maintain the pH at 7 in thermal denaturation assay of purified protein[3]
• In isocratic elution of protein using gel filtration[3]
• Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy[3]

General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[4] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[5]

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable