The solubility of this product has been tested in ethanol at 1 mg/mL, and heating to 37°C may be required for complete solubilization. The solubility in DMSO has not been tested, but various sources indicate that this product is soluble in DMSO at a minimum of 20 mg/mL.
H0135
Hydrocortisone
γ-irradiated, powder, BioXtra, suitable for cell culture
Synonym(s):
11β,17α,21-Trihydroxypregn-4-ene-3,20-dione, 17-Hydroxycorticosterone, 4-Pregnene-11β,17α,21-triol-3,20-dione, Cortisol, Kendall’s compound F, Reichstein’s substance M
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About This Item
Empirical Formula (Hill Notation):
C21H30O5
CAS Number:
Molecular Weight:
362.46
Beilstein:
1354819
EC Number:
MDL number:
UNSPSC Code:
12352209
PubChem Substance ID:
NACRES:
NA.77
Biological source:
synthetic (organic)
Form:
powder
Assay:
≥80% (HPLC)
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biological source
synthetic (organic)
Quality Level
sterility
γ-irradiated
product line
BioXtra
Assay
≥80% (HPLC)
form
powder
technique(s)
cell culture | mammalian: suitable
mp
211-214 °C (lit.)
solubility
ethanol: 1 mg/mL, clear
shipped in
ambient
storage temp.
room temp
SMILES string
[H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO
InChI
1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
InChI key
JYGXADMDTFJGBT-VWUMJDOOSA-N
Gene Information
human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)
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This Item | H0888 | H3160 | H6909 |
|---|---|---|---|
| biological source synthetic (organic) | biological source soybean | biological source plant (wild yam) | biological source non-animal source |
| assay ≥80% (HPLC) | assay ≥98% | assay 97.0-102.0% dry basis | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 500 |
| product line BioXtra | product line BioReagent | product line - | product line BioXtra |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable |
| form powder | form powder | form powder | form liquid |
General description
Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.
Application
Hydrocortisone is used for the following applications:
- Used in myogenic induction and identification[1]
- Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)[2]
- Used in adipogenic differentiation (one of the components in adipogenic medium)[3]
- Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components[4]
- The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone[5]
- Used in differentiation assays of melanoma spheroid cells[6]
Biochem/physiol Actions
Primary glucocorticoid secreted by the adrenal cortex.
Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.
Physical form
powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw
Preparation Note
To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.
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Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Repr. 1A - STOT RE 2
Target Organs
Adrenal gland,Bone marrow
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Dong Fang et al.
Cancer research, 65(20), 9328-9337 (2005-10-19)
Recent studies suggest that cancer can arise from a cancer stem cell (CSC), a tumor-initiating cell that has properties similar to those of stem cells. CSCs have been identified in several malignancies, including those of blood, brain, and breast. Here
Q L Hao et al.
Blood, 86(10), 3745-3753 (1995-11-15)
We present cell cycling and functional evidence that the CD34+CD38- immunophenotype can be used to define a rare and primitive subpopulation of progenitor cells in umbilical cord blood. CD34+CD38- cells comprise 0.05% +/- 0.08% of the mononuclear cells present in
Andrej Jedinak et al.
International journal of oncology, 33(6), 1307-1313 (2008-11-21)
In spite of the global consumption of mushrooms, only two epidemiological studies demonstrated an inverse correlation between mushroom intake and the risk of cancer. Therefore, in the present study we evaluated whether extracts from edible mushrooms Agaricus bisporus (portabella), Flammulina
Oscar K Lee et al.
Blood, 103(5), 1669-1675 (2003-10-25)
It is well accepted that umbilical cord blood has been a source for hematopoietic stem cells. However, controversy exists as to whether cord blood can serve as a source of mesenchymal stem cells, which can differentiate into cells of different
K L van Golen et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 5(9), 2511-2519 (1999-09-28)
Inflammatory breast cancer is a rapidly growing, distinct form of locally advanced breast cancer that carries a guarded prognosis. To identify the genes that contribute to this aggressive phenotype, we compared under- and overexpressed sequences in an inflammatory breast tumor
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Could I use DMSO as the solvent for hydrocortisone ?
1 answer-
Helpful?
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How long is the 50 μg/mL stock solution (1 mL absolute ethanol + 19 mL sterile medium) stable when stored in frozen aliquots at -20°C?
1 answer-
Unfortunately, the solution stability of this product at -20°C is not determined internally. However, various sources suggest frozen aliquots stored at -20°C are stable for at least 3-4 months. It is strongly recommend to protect the solution from light.
Helpful?
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What are the recommended storage conditions for stock solutions of item H0135, Hydrocortisone?
1 answer-
The stock solutions can be stored at 4°C and are stable for up to 1 month.
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