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FLAGIPT1

Millipore

FLAG® Immunoprecipitation Kit

All Photos(1)
Synonym(s):
Anti-ddddk, Anti-dykddddk
NACRES:
NA.32

usage

 kit sufficient for 50 reactions

Quality Level

300

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

storage temp.

−20°C

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This Item
M8823F2426F3290
FLAG® Immunoprecipitation Kit

Millipore

FLAGIPT1

FLAG® Immunoprecipitation Kit

Anti-FLAG® M2 Magnetic Beads affinity isolated antibody

Millipore

M8823

Anti-FLAG® M2 Magnetic Beads

EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2

Millipore

F2426

EZview™ Red ANTI-FLAG® M2 Affinity Gel

FLAG® Peptide lyophilized powder

Millipore

F3290

FLAG® Peptide

technique(s)

immunoprecipitation (IP): suitable

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

-

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

300

Quality Level

-

Quality Level

-

Quality Level

200

analyte chemical class(es)

proteins (FLAG® Affinity Gels)

analyte chemical class(es)

proteins (FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag)

analyte chemical class(es)

proteins (FLAG® Affinity Gels, FLAG® tag, 3xFLAG® tag, DYKDDDDK tag)

analyte chemical class(es)

-

General description

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Browse additional application references in our FLAG® Literature portal.

Features and Benefits

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3X FLAG™ peptide provides efficient and gentle elution through direct competition

Other Notes

All prodedures to be performed at 2-8°C.

Legal Information

3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • A2220ANTI-FLAG M2 affinity gel 1 mLSDS
  • SAE01943X FLAG peptide 1 mgSDS

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319 - H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Samer Swedan et al.
Journal of virology, 85(19), 10090-10100 (2011-07-29)
Human respiratory syncytial virus (RSV), a major cause of severe respiratory diseases, efficiently suppresses cellular innate immunity, represented by type I interferon (IFN), using its two unique nonstructural proteins, NS1 and NS2. In a search for their mechanism, NS1 was
Mateusz Wydro et al.
Nucleic acids research, 38(11), 3732-3742 (2010-02-11)
Mammalian mitochondria contain their own genome that is almost fully transcribed from both strands, generating polycistronic RNA units that are processed and matured. The mitochondrial mRNA is modified by oligo- or polyadenylation at the 3' termini, but the exact function
Andrew S Terker et al.
American journal of physiology. Renal physiology, 315(4), F781-F790 (2018-02-08)
With no lysine kinase 4 (WNK4) is essential to activate the thiazide-sensitive NaCl cotransporter (NCC) along the distal convoluted tubule, an effect central to the phenotype of familial hyperkalemic hypertension. Although effects on potassium and sodium channels along the connecting
Kuancan Liu et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(3), 901-913 (2020-01-29)
Esophageal squamous cell carcinoma (ESCC) is a predominant cancer type in developing countries such as China, where ESCC accounts for approximately 90% of esophageal malignancies. Lacking effective and targeted therapy contributes to the poor 5-year survival rate. Recent studies showed
Kalpana Kannan et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(11), E1272-E1277 (2015-03-04)
High-grade serous ovarian cancer (HGSC) is among the most lethal forms of cancer in women. Excessive genomic rearrangements, which are expected to create fusion oncogenes, are the hallmark of this cancer. Here we report a cancer-specific gene fusion between BCAM
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