1.01299

Enterokoki 100

ReadyCult^®, granular ( ), for Enterococcus spp., pack of 20 tests snap packs

• NACRES: NA.74
• UNSPSC Code: 41106212
• Manufacturer/tradename: ReadyCult^®
• Ph: 7.3-7.7 (25 °C, 17.8 g/L in H₂O, after autoclaving)
• Form: granular ( )
• Suitability: Enterococcus spp.
• Application(s): food and beverages; microbiology

Właściwości

• Quality Segment: 200
• sterility: sterile; irradiated
• product line: ReadyCult^®
• form: granular ( )
• packaging: pack of 20 tests snap packs
• manufacturer/tradename: ReadyCult^®
• technique(s): microbiological culture: suitable
• pH: 7.3-7.7 (25 °C, 17.8 g/L in H₂O, after autoclaving)
• solubility: 17.8 g/L
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Enterococcus spp.

Opis

General description

Enterococci 100 medium is designed for the selective detection of enterococci and D-streptococci, which are key indicators of fecal contamination. The medium contains sodium azide, which suppresses the growth of many accompanying microorganisms, particularly Gram-negative bacteria, while allowing enterococci to grow. It also includes the chromogenic substrate X-GLU (5-bromo-4-chloro-3-indolyl-ß-D-glucopyranoside). This substrate is cleaved by ß-D-glucosidase, an enzyme characteristic of enterococci, resulting in an intense blue-green color. This color change provides a clear visual indication of the presence of enterococci and D-streptococci.

Application

Enterococci 100 medium is used for the detection and confirmation of enterococci and D-streptococci in water samples. It is particularly used in food and beverage testing for assessing fecal contamination in drinking water, process water, environmental water, and other water sources where microbiological quality must be monitored. The selective and chromogenic properties of the medium support rapid, easy-to-read results and help reduce false positives from other ß-D-glucosidase-positive bacteria.

Analysis Note

Wygląd
- klarowność:
klarowny
- kolor: żółtawy do żółtawo-brązowego
Wartość pH (25 °C): 7,3 - 7,7
Inokulum na podłożu referencyjnym
- Enterococcus faecalis ATCC 19433 (WDCM 00009):
10 - 100
- Enterococcus durans ATCC 19432: 10 - 100
- Staphylococcus aureus ATCC 25923 (WDCM 00034): ≥ 1000
Wzrost
- Enterococcus faecalis ATCC 19433 (WDCM 00009):
dość dobry do dobrego
- Enterococcus durans ATCC 19432: dość dobry do dobrego
- Staphylococcus aureus ATCC 25923 (WDCM 00034): dość dobry do dobrego
Zmiana koloru na zielono-niebieski
- Enterococcus faecalis ATCC 19433 (WDCM 00009):
+
- Enterococcus durans ATCC 19432: +
- Staphylococcus aureus ATCC 25923 (WDCM 00034): -
Inkubacja: 24 - 48 godzin; 35 °C; warunki tlenowe.

Other Notes

Zastępuje: 1.01299.0002

Legal Information

READYCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06,GHS09
• Słowo ostrzegawcze: Danger
• Ostrzeżenia: P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2
• supp_hazards: EUH032
• Kody zagrożeń: H302 + H332,H311,H411
• Klasa składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects