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Informacje o tej pozycji
biological source
human
recombinant
expressed in E. coli
product line
Upstate®
specific activity
(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
mol wt
Mw 30 kDa (AMPKβ1), Mw 38 kDa (AMPKγ1), Mw 63 kDa (AMPKα2)
purified by
(Ni2+/NTA-agarose)
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
accession no.
(Function: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5′-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. ), NM_006252
UniProt accession no.
relevant disease(s)
diabetes; obesity; metabolic disorders
Gene Information
human ... PRKAA2(5563), PRKAB1(5563)
General description
Application
Metabolism
Apoptosis & Cancer
Diabetes
Obesity
Metabolic Disorders
Biochem/physiol Actions
Packaging
Physical form
Preparation Note
Analysis Note
Other Notes
Legal Information
Disclaimer
1 of 1
Ta pozycja | |||
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| description Active, recombinant, human AMPK (α2, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα2, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays. | description Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays. | description PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution | description PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution |
| biological source human | biological source human | biological source - | biological source human |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in baculovirus infected Sf9 cells | recombinant expressed in baculovirus infected Sf9 cells |
| technique(s) activity assay: suitable (kinase) | technique(s) activity assay: suitable (kinase) | technique(s) - | technique(s) - |
| mol wt Mw 63 kDa (AMPKα2), Mw 38 kDa (AMPKγ1), Mw 30 kDa (AMPKβ1) | mol wt Mw 30 kDa (AMPKβ1), Mw 38 kDa (AMPKγ1), Mw 64 kDa (AMPKα1) | mol wt ~40 kDa (G1), ~69 kDa (A2), ~36 kDa (B2) | mol wt G1 subunit ~40 kDa, B1 subunit ~38 kDa, A1 subunit ~68 kDa |
| specific activity (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.) | specific activity - | specific activity 238-322 nmol/min·mg | specific activity 626-848 nmol/min·mg |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. |
Słowo ostrzegawcze
Warning
Kody zagrożeń
Hazard Classifications
Skin Sens. 1
Klasa składowania
10 - Combustible liquids
WGK
WGK 1
Temperatura zapłonu (°C)
Not applicable
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