178273

Aphidicolin

≥98% (HPLC), solid, DNA polymerase α and δ inhibitor, Calbiochem

• Synonim(y): Aphidicolin
• Wzór empiryczny (zapis Hilla): C₂₀H₃₄O₄
• Numer CAS: 38966-21-1
• Masa cząsteczkowa: 338.48
• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD00083214
• Assay: ≥98% (HPLC)
• Form: solid
• Storage condition: OK to freeze

Właściwości

• Nazwa produktu: Aphidicolin, Aphidicolin, CAS 38966-21-1, is a cell-permeable antibiotic that acts as a cell synchronization agent. Blocks the cell cycle at early S-phase.
• Quality Segment: 100
• description: Merck USA index - 14, 727
• assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: DMSO: 50 mg/mL, ethanol: soluble, methanol: soluble
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: O[C@]1([C@H]2C[C@@]3([C@@]4([C@H]([C@@]([C@@H](CC4)O)(CO)C)CC[C@H]3C2)C)CC1)CO
• InChI: 1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1
• InChI key: NOFOAYPPHIUXJR-APNQCZIXSA-N

Opis

General description

A cell-permeable tetracyclic diterpene antibiotic that acts as a cell synchronization agent. Blocks the cell cycle at early S-phase. Specific inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses. Potentiates apoptosis induced by arabinosyl nucleosides in leukemia cell lines. Also induces apoptosis in HeLa S3 cells, but inhibits vincristine-induced apoptosis in the p53-negative human prostate cancer cell line PC-3. Also available as a 30 mM solution in DMSO (Cat. No. 504744).

Biochem/physiol Actions

Cell permeable: yes

Primary Target
DNA polymerase α, DNA polymerase δ

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Other Notes

Borner, M.M., et al. 1995. Cancer Res.55, 2122.
Poluha, W., et al. 1995. Oncogene 10, 185.
Urbani, L., et al. 1995. Exp. Cell Res. 219, 159.
Schimke, R.T., et al. 1994. Philos. Trans. R. Soc. London. B. Biol. Sci.345, 311.
Kuwakado, K., et al. 1993. Biochem. Pharmacol. 46, 1909.
Hubermann, J.A. 1981. Cell23, 647.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable