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EGTA, Molecular Biology Grade

Synonim(y):

EGTA, Molecular Biology Grade, Ethyleneglycol- bis(β-aminoethyl)-N,N,Nʹ,Nʹ-tetraacetic Acid

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Rozmiar/SKUDostępnośćCena netto
25 g

Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o.

1090,00 zł

Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C14H24N2O10
Numer CAS:
Masa cząsteczkowa:
380.35
UNSPSC Code:
12352106
NACRES:
NA.25
MDL number:

1090,00 zł


Dostępne do wysyłki DZISIAJSzczegóły


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grade

Molecular Biology

Quality Segment

description

RTECS - AH3760000

assay

≥98% dry basis (complexometric)

form

solid (fine)

reaction suitability

reagent type: chelator

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

1 M NaOH: soluble

cation traces

heavy metals (as Pb): ≤0.001%

foreign activity

DNases, proteases, RNases, none detected

shipped in

ambient

storage temp.

10-30°C

SMILES string

N(CCOCCOCCN(CC(=O)O)CC(=O)O)(CC(=O)O)CC(=O)O

InChI

1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)

InChI key

DEFVIWRASFVYLL-UHFFFAOYSA-N

General description

Chelating agent selective for Ca2+. Binds one cation per molecule. Induces apoptosis in pheochromocytoma (PC12) cells.

Application


  • Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.: This study presents a genetically encoded biosensor for real-time monitoring of lysosomal pH changes, potentially enhanced by buffering agents like EGTA. The findings may offer insights into cellular mechanisms influenced by calcium chelation (Ponsford et al., 2021).

  • Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca(2+).: The research highlights the role of calcium in fungal development, with EGTA potentially influencing these biological processes through calcium chelation (Zhu et al., 2019).


Preparation Note

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 1 year at 4°C.

Other Notes

Lindenboim, L., et al. 1995. J. Neurochem.64, 1054.
Bers, D.M. 1982. Am. J. Physiol.242, C404.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)
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03777E4378E3889
description

-

description

BioUltra, ≥99.0% (T)

description

≥97.0%

description

Molecular Biology, ≥97.0%

grade

Molecular Biology

grade

Molecular Biology

grade

-

grade

Molecular Biology

assay

≥98% dry basis (complexometric)

assay

≥99.0% (T)

assay

≥97.0%

assay

≥97.0%

Quality Level

100

Quality Level

300

Quality Level

400

Quality Level

400

form

solid (fine)

form

powder

form

powder

form

powder

storage temp.

10-30°C

storage temp.

-

storage temp.

room temp

storage temp.

-

solubility

1 M NaOH: soluble

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

solubility

1 M NaOH: 1.1 g/10 mL, clear, colorless

solubility

-


Klasa składowania

11 - Combustible Solids

WGK

WGK 2



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Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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