324630

EHNA, Hydrochloride

≥97% (HPLC), PDE II-selective phosphodiesterase inhibitor, solid

• Synonim(y): EHNA, Hydrochloride, erythro-9-(2-Hydroxy-3-nonyl)adenine, HCl
• Wzór empiryczny (zapis Hilla): C₁₄H₂₃N₅O · xHCl
• Numer CAS: 51350-19-7
• Masa cząsteczkowa: 277.37 (free base basis)
• MDL number: MFCD00151066
• UNSPSC Code: 12352200
• NACRES: NA.77
• eCl@ss: 32150313
• Assay: ≥97% (HPLC)
• Form: solid
• Storage condition: OK to freeze, desiccated (hygroscopic)

Właściwości

• Nazwa produktu: EHNA, Hydrochloride, The racemic mixture of a cell-permeable adenine derivative that acts as a PDE II-selective phosphodiesterase inhibitor and adenosine deaminase.
• Quality Segment: 100
• assay: ≥97% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic)
• color: white
• solubility: water: 25 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: [n]1(c2ncnc(c2nc1)N)[C@H]([C@@H](O)C)CCCCCC
• InChI: 1S/C14H23N5O/c1-3-4-5-6-7-11(10(2)20)19-9-18-12-13(15)16-8-17-14(12)19/h8-11,20H,3-7H2,1-2H3,(H2,15,16,17)/t10-,11-/m0/s1
• InChI key: IOSAAWHGJUZBOG-QWRGUYRKSA-N

Opis

General description

The racemic mixture of a cell-permeable adenine derivative that acts as a PDE II-selective phosphodiesterase inhibitor (IC50 = 0.8 and 2 µM against human and procine PDE II, respectively) and adenosine deaminase (K_i = 4 nM), while exhibiting little or much reduced potency toward PDE I/II/IV isozymes (IC50 >100 µM) of both human and porcine species. EHNA at 10 µM concentration is also reported to effectively and reversibly block the differentiation and maintain the pluripotency of hES (human embryonic stem) cell line SA121 in feeder-free cultures without exogenously added FGF for up to 30 passages in a manner independent of its known ADA and PDE inhibitory activities.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
PDE 2

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 0.8 and 2 µM against human and procine PDE II, respectively

Target K_i: 4 nM against adenosine deaminase

Other Notes

Burton, P., et al. 2010. Biochem. J.in press.
Lorbar, M., et al. 1999. J. Mol. Cell. Cardiol.31, 401.
Verde, I., et al. 1999. Br. J. Pharmacol.127, 65.
Reynolds, A.J., et al. 1996. Brain Res.798, 67.
Mery, P.F., et al. 1995. Mol. Pharmacol.48, 121.
Podzuweit, T., et al. 1995. Cell. Signal.7, 733.
Bessodes, M., et al. 1982. Biochem. Pharmacol.31, 879.
Bouchard, P., et al. 1981. Proc. Natl. Acad. Sci. USA78, 1033.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 11 - Combustible Solids
• WGK: WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable