This product is lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.
Our recommendation is to further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H?O.
https://b2b.sigmaaldrich.com/US/en/product/mm/475911#product-documentation
475911
Myeloperoxidase, Human Polymorphonuclear Leukocytes
A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins.
Synonim(y):
Myeloperoxidase, Human Polymorphonuclear Leukocytes, MPO
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Informacje o tej pozycji
Numer CAS:
Numer WE:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.25
Specific activity:
150-200 units/mg protein
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Informacje o cenach i dostępności nie są obecnie dostępne.
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Pozwól nam pomócbiological source
human
Quality Segment
assay
≥95% (SDS-PAGE)
form
lyophilized
specific activity
150-200 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
water: soluble (volume is lot-specific and can be found on the product label)
shipped in
wet ice
storage temp.
−20°C
SMILES string
[O+H2]O[O-]
InChI
1S/H2O3/c1-3-2/h1-2H
InChI key
JSPLKZUTYZBBKA-UHFFFAOYSA-N
General description
Native, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.
Physical form
Lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.
Further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H₂O.
Other Notes
Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.
One unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Ta pozycja | |||
|---|---|---|---|
| description A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. | description lyophilized powder, ≥50 units/mg protein | description lyophilized powder (essentially salt-free), ≥200 units/mg protein | description lyophilized, powder, ≥150 U/mg |
| assay ≥95% (SDS-PAGE) | assay - | assay - | assay - |
| biological source human | biological source human leucocytes | biological source bovine milk | biological source - |
| specific activity 150-200 units/mg protein | specific activity ≥50 units/mg protein | specific activity ≥200 units/mg protein | specific activity ≥150 U/mg |
| form lyophilized | form lyophilized powder | form lyophilized powder (essentially salt-free) | form powder |
| shipped in wet ice | shipped in dry ice | shipped in - | shipped in wet ice |
| storage condition OK to freeze | storage condition - | storage condition - | storage condition - |
Słowo ostrzegawcze
Danger
Kody zagrożeń
Ostrzeżenia
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
WGK
WGK 1
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Dear Tecnical Support, How should I reconstitute this product?Would it be fine that I reconstitute it by adding 100 microliter of deionized water, so that I obtain a 1mg/mL of protein in the original buffer? Then further dilute it in the indicated buffer?
1 answer-
Helpful?
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