71077
X-Gal Solution - Novagen
Convenience for blue/white screening
Synonim(y):
X-Gal solution
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
3 mL | Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o. | 658,00 zł |
Informacje o tej pozycji
NACRES:
NA.54
UNSPSC Code:
12352200
658,00 zł
Dostępne do wysyłki DZISIAJSzczegóły
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócQuality Segment
form
solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
tissue culture: suitable
shipped in
wet ice
storage temp.
−20°C
General description
X-Gal, or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is an organic compound commonly used in molecular biology as a chromogenic substrate for the enzyme β-galactosidase, which is encoded by the LacZ gene. When β-galactosidase hydrolyzes X-Gal, it forms galactose and 5,5′-dibromo-4,4′-dichloro-indigo, resulting in the formation of intense blue color colonies. This process is widely used in LacZ activity assays and staining techniques, such as X-gal staining or LacZ staining to distinguish between recombinants (white) and nonrecombinants (blue). X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing. X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO, ready for dilution into the culture medium or appropriate buffers.
Application
X-Gal Solution - Novagen has been used at 1:40 dilution to detect the expression of β-galactosidase and mutant fusion protein generated by the ApcΔ242 mutation in the tissue sample.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description Convenience for blue/white screening | description - | description Novagen′s pET-9a-d(+) vectors carry an N-terminal T7•Tag sequence and BamH I cloning site. | description - |
| technique(s) tissue culture: suitable | technique(s) - | technique(s) - | technique(s) - |
| form solution | form liquid | form - | form - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| shipped in wet ice | shipped in wet ice | shipped in dry ice, wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −70°C |
| storage condition OK to freeze | storage condition OK to freeze | storage condition OK to freeze, avoid repeated freeze/thaw cycles | storage condition OK to freeze, avoid repeated freeze/thaw cycles |
Klasa składowania
10 - Combustible liquids
Co się dzieje?
WGK 3
Temperatura zapłonu (°F)
188.6 °F
Temperatura zapłonu (°C)
87 °C
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.