The band detected on western blot is typically between 100-120 kDa. Please see a link to the reference below.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7425076/#MOESM2
AB5302
Anti-Amyloid Precursor Protein (APP) Antibody
CHEMICON®, rabbit polyclonal
Synonim(y):
APP
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| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
100 μL | Przewidywany termin wysyłki19 października 2026zKuehne + Nagel Sp. z o.o. | 2130,00 zł |
Informacje o tej pozycji
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
monkey, mouse, human, bovine
Application:
ICC, IHC, IP, WB
Citations:
7
2130,00 zł
Przewidywany termin wysyłki19 października 2026Szczegóły
Pomoc techniczna
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Pozwól nam pomócNazwa produktu
Anti-Amyloid Precursor Protein Antibody, KPI Domain, serum, Chemicon®
biological source
rabbit
Quality Segment
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
monkey, mouse, human, bovine
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... APP(351)
Immunogen
APP.
Application
Anti-Amyloid Precursor Protein Antibody, KPI Domain is an antibody against Amyloid Precursor Protein for use in IP, WB, IC, IH.
Western blot: 1:250 - 1:1,000
Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.
Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.
Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.
Optimal working dilutions must be determined by end user.
Reduction /Alkylation protocol:
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.
Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.
Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.
Optimal working dilutions must be determined by end user.
Reduction /Alkylation protocol:
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Biochem/physiol Actions
Amyloid Precursor Protein (APP), KPI Domain.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Ta pozycja | |||
|---|---|---|---|
| description serum, Chemicon® | description clone 4.1, Chemicon®, from mouse | description serum, Chemicon® | description clone OM84, Chemicon®, from mouse |
| biological source rabbit | biological source mouse | biological source rabbit | biological source mouse |
| antibody form serum | antibody form purified immunoglobulin | antibody form serum | antibody form purified immunoglobulin |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| clone polyclonal | clone 4.1, monoclonal | clone polyclonal | clone OM84, monoclonal |
| species reactivity monkey, mouse, human, bovine | species reactivity human | species reactivity human, monkey | species reactivity human |
| shipped in dry ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
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Hello, I bought this antibody and I run wstern-blot. I would like to know what is the molecular wight for the KPI domain? Thanks
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