BioTracker ATP-Red Live Cell Dye is a fluorescent probe that increases emission upon binding ATP, with mitochondrial localization commonly observed due to ATP abundance. The product documentation does not explicitly confirm behavior with FCCP or CCCP. Uncouplers disrupt mitochondrial function and ATP levels, potentially affecting the probe signal; evaluation with FCCP/CCC P has not been performed for this product. Therefore, use should be interpreted cautiously. A literature reference describing similar ATP-targeting probes is Chang YT et al., Angew. Chem. Int. Ed. Engl. 2016;55(5):1773–1776.
Wybierz wielkość
| Rozmiar/SKU | Dostępność | Cena netto |
|---|---|---|
1 mg | Przewidywany termin wysyłki02 listopada 2026zKuehne + Nagel Sp. z o.o. | 1930,00 zł |
Informacje o tej pozycji
1930,00 zł
Przewidywany termin wysyłki02 listopada 2026Szczegóły
assay
≥98% (H-NMR), ≥98% (HPLC), ≥98% (LC/MS), ≥98% (elemental analysis)
technique(s)
cell based assay: suitable, live cell imaging : suitable
detection method
fluorometric
shipped in
ambient
storage temp.
-10 to -25°C
General description
The BioTracker ATP-Red dye is a live cell red fluorescent imaging probe for adenosine triphosphate (ATP). The probe targets ATP specifically in the mitochondria of living cells. The probe shows no cross reactivity to numerous analytes including: Zn2+, Mg2+, Ca2+, Na2+, K+, GSH, HOCL, H2O2, arabinose, galactose, glucose, fructose, ribose, sorbose, sucrose, xylose, heparin, AMP, ADP, CMP, CDP, CTP, UMP, UDP, UTP, GMP, GDP or GTP. The probe is non-fluorescent when forming a closed ring structure. In the presence of the negatively charged ATP, the covalent bonds between boron and ribose is broken and the ring opens producing fluorescence.
Spectral Properties
Excitation max: 565 nm
Emission max: 592 nm
Application
Cell Imaging
Live Cell Dye
Physical form
Preparation Note
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analysis Note
Molar Mass: 561.48 g/mol
Disclaimer
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description Live cell imaging dye for cellular adenosine triphosphate (ATP) localized to mitochondria used to detect metabolic activity, cell proliferation and overall cell health. | description Live cell imaging dye for alkaline phosphatase (AP) for cancer and stem cell applications. | description Live cell imaging dye for hypochlorus acid (HOCL) used to detect phagocytosis, inflammation and cellular apoptosis. | description Live Cell imaging probe which reversibly fluoresces in acidic pH, such as in lysosomes and endosomes of cells |
| technique(s) cell based assay: suitable, live cell imaging : suitable | technique(s) live cell imaging : suitable, cell based assay: suitable | technique(s) cell based assay: suitable, live cell imaging : suitable | technique(s) cell based assay: suitable |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in dry ice |
| assay ≥98% (HPLC), ≥98% (elemental analysis), ≥98% (H-NMR), ≥98% (LC/MS) | assay ≥98% (H-NMR), ≥98% (elemental analysis), ≥98% (LC/MS), ≥98% (HPLC) | assay ≥98% (H-NMR), ≥98% (LC/MS), ≥98% (elemental analysis), ≥98% (HPLC) | assay ≥80% (LC) |
| storage temp. -10 to -25°C | storage temp. -10 to -25°C | storage temp. -10 to -25°C | storage temp. −20°C |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method - |
Klasa składowania
11 - Combustible Solids
WGK
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Can BioTracker ATP-Red Live Cell Dye be used with an uncoupler like FCCP/CCCP? Mitochondrial localization of molecules is often related to the weak negative charge in the matrix that is negated by uncouplers which would affect conclusions.
1 answer-
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SCT045 BioTracker ATP-Red Live Cell Dye does it work on cell lysates?
1 answer-
SCT045 BioTracker ATP-Red Live Cell Dye is not suitable for use in cell lysates as it is intended for live cell applications.
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