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TMS-010

Alcian-Blue Staining Solution

Synonim(y):

Alcian blue 8G, Alcian blue PAS stain, Alcian blue stain

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Rozmiar/SKUDostępnośćCena netto
100 mL

Dostępne do wysyłki DZISIAJzKuehne + Nagel Sp. z o.o.

543,00 zł

Informacje o tej pozycji

NACRES:
NA.75
UNSPSC Code:
12352207

543,00 zł

Dostępne do wysyłki DZISIAJSzczegóły

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Quality Segment

technique(s)

cell based assay: suitable, cell culture | stem cell: suitable, cell differentiation: suitable

General description

Alcian blues, are a family of polyvalent basic dyes, of which, the Alcian blue 8G has been historically the most common and the most reliable member. It is used to stain acidic polysaccharides such as glycosaminoglycans in cartilages and other body structures, some types of mucopolysaccharides, sialylated glycocalyx of cells etc. Alcian blue if often used to identify Chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells.

Application

Research Category
Stem Cell Research
Research Sub Category
Osteobiology

Arthritis

Physical form

Product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.
Product is presented as a 1% w/v Alcian Blue solution.

Preparation Note

Store at room temperature for up to 4 months from date of receipt. If Alcian-Blue solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.

Analysis Note

Solution is blue with no particulates present. Solution is suitable for endpoint cell culture assays.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Co się dzieje?

WGK 2

Piktogramy

FlameCorrosion
GHS02,GHS05

Słowo ostrzegawcze

Danger

Kody zagrożeń

H226,H314

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 3 - Skin Corr. 1A

Klasa składowania

3 - Flammable liquids

Temperatura zapłonu (°F)

102.2 °F - closed cup

Temperatura zapłonu (°C)

39 °C - closed cup

Wykazy regulacyjne

Wykazy regulacyjne dotyczą głównie produktów chemicznych. Można w nich podawać ograniczoną liczbę informacji na temat produktów niechemicznych. Brak wpisu oznacza, że żaden ze składników nie znajduje się w wykazie. Użytkownik odpowiada za zagwarantowanie bezpiecznego i zgodnego z prawem stosowania produktu.

64-19-7

CAS No.

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  

  1. What is the composition of the differentiation for TMS 010 C procedure?

    1 answer

    1. Product TMS-010 is a standard pH 2.5 solution of Alcian Blue that stains a number of substances, including chondrocytes, at this pH. The provided procedure is similar but not identical to the standard histology stain procedure found in most histology textbooks.

      Helpful?

  2. Is there a procedure available for using TMS-010, and in this context, would you anticipate the alcian blue to be present in the nuclei, cytoplasm, or the entire cell?

    1 answer

    1. Depending on the preparation of Alcian Blue, the dye can be used to identify various substances. TMS-010 seems to have been formulated to stain chondrocytes, the cells that produce collagen. It's expected that the staining should be primarily in the cytoplasm. However, if the staining intensity is high, it might appear that the entire cell is stained.

      Helpful?

  3. What is the concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product?

    1 answer

    1. The concentration of acetic acid specified for the Alcian Blue pH 2.5 TMS-010-C product is not provided. However, it is a classical textbook formulation typically consisting of 1% Alcian Blue in 3% glacial acetic acid.

      Helpful?

  4. Can you provide a protocol for a colorimetric assay that uses Alcian Blue to compare sulfated proteoglycan content in adherent cancer cell lines?

    1 answer

    1. The Alcian-Blue Staining Solution (cat. no. TMS-010-C) is a cell culture product from the cell culture portfolio, intended for cell-based assays, cell differentiation, and stem cell culture. For example, to identify chondrocytes from differentiated cultures of both human and rodent mesenchymal stem cells (MSCs).

      This product is presented as a 1% w/v Alcian Blue solution in acetic acid at pH 2.5.

      The Certificate of Analysis does not include any usage instructions and there is no separate product data sheet or user guide.

      The following paper may be helpful for the staining of stem cells:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432525/

      Helpful?

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