biological source
bovine (calf) intestine
form
lyophilized
mol wt
150 kDa
packaging
pkg of 3 × 250 μg (11351311001), pkg of 3 × 30 μg (11334115001)
manufacturer/tradename
Roche
concentration
1:50 % (w/w)
optimum pH
8
storage temp.
2-8°C
General description
Enterokinase is a protease from calf intestine and supplied in a quality optimized for the cleavage of fusion proteins.
Enterokinase is a heterodimeric serine protease involved in the conversion of trypsinogen to trypsin. It cleaves trypsinogen by highly specific manner at the trypsinogen activation peptide site following the sequence (A~p)~-L and stimulates its physiological activities.
Enterokinase is a heterodimeric serine protease involved in the conversion of trypsinogen to trypsin. It cleaves trypsinogen by highly specific manner at the trypsinogen activation peptide site following the sequence (A~p)~-L and stimulates its physiological activities.
Application
Quality
Purity: highly purified, not standardized with albumin
Quality control: function tested
Purity: highly purified, not standardized with albumin
Quality control: function tested
Enterokinase is used for the cleavage of fusion proteins at definite cleavage sites. For the processing of recombinant proteins, the desired protein is fused with Enterokinase recognition sequence. After purification of the entire fusion protein, the protein or peptide is released by incubation with enterokinase.
Biochem/physiol Actions
Serine protease acting as a restriction protease that recognizes the amino acid sequence -(Asp)4-Lys-X. The aspartic acid residues can be partially substituted by glutamic acid.
Preparation Note
Working concentration: 1:50 (w/w)
Storage conditions (working solution): A solution of enterokinase, stored at 2 to 8 °C, can be used up to one week.
Storage conditions (working solution): A solution of enterokinase, stored at 2 to 8 °C, can be used up to one week.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Słowo ostrzegawcze
Danger
Kody zagrożeń
Hazard Classifications
Resp. Sens. 1
Klasa składowania
11 - Combustible Solids
Co się dzieje?
WGK 1
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
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