Wszystkie zdjęcia(1)

93595

Sigma-Aldrich

Trypan Blue solution

0.4%, for microscopy

Empirical Formula (Hill Notation):
C34H24N6Na4O14S4
Numer CAS:
Masa cząsteczkowa:
960.81
Beilstein:
4360496
Numer MDL:
Identyfikator substancji w PubChem:
NACRES:
MA.02

Poziom jakości

200

klasa czystości

for microscopy

forma

liquid

okres trwałości

limited shelf life, expiry date on the label

stężenie

0.4%

gęstość

1.007 g/mL at 20 °C

εmax

1.4 at 603  nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

temp. przechowywania

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

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Opis ogólny

Trypan Blue is a tetrasulfonated anionic, hydrophilic dye.

Zastosowanie

Trypan blue is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents. It is also employed as a collagen stain in a Van Gieson procedure. To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.

Działania biochem./fizjol.

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

Postać fizyczna

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

piktogramy

Health hazard

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Carc. 1B

Kod klasy składowania

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certyfikat analizy

Świadectwo pochodzenia

Sheetal A Thakur et al.
Toxicological sciences : an official journal of the Society of Toxicology, 107(1), 238-246 (2008-10-07)
Alveolar macrophages (AM) in the lung have been documented to play pivotal roles in inflammation and fibrosis (silicosis) following inhalation of crystalline silica (CSiO(2)). In contrast, exposure to either titanium dioxide (TiO(2)) or amorphous silica (ASiO(2)) is considered relatively benign.
Ulrich Putz et al.
Science signaling, 5(243), ra70-ra70 (2012-09-27)
Exosomes are microvesicles of endosomal origin that are secreted, and their contents (proteins, lipids, DNA, or microRNAs) can alter the physiological states of recipient cells. We demonstrated that phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor
Marco Todesco et al.
PLoS genetics, 10(7), e1004459-e1004459 (2014-07-11)
A fundamental question in biology is how multicellular organisms distinguish self and non-self. The ability to make this distinction allows animals and plants to detect and respond to pathogens without triggering immune reactions directed against their own cells. In plants
Mehlika Hazar-Rethinam et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 21(2), 417-427 (2014-11-21)
Head and neck squamous cell carcinomas (HNSCC) are frequently drug resistant and have a mortality rate of 45%. We have previously shown that E2F7 may contribute to drug resistance in SCC cells. However, the mechanism and pathways involved remain unknown.
Georgia E Hodes et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(45), 16136-16141 (2014-10-22)
Depression and anxiety disorders are associated with increased release of peripheral cytokines; however, their functional relevance remains unknown. Using a social stress model in mice, we find preexisting individual differences in the sensitivity of the peripheral immune system that predict

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