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B3883

Bile bovine

dried, unfractionated

Synonim(y):

Ox gall powder

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Rozmiar/SKUDostępnośćCena netto
25 g
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
269,00 zł
100 g
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
796,00 zł
500 g
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1860,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
41106212
EC Number:
232-369-0
NACRES:
NA.85
MDL number:

269,00 zł


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sterility

non-sterile

Quality Segment

form

dried, unfractionated

color

yellow-brown

solubility

water: soluble 50 mg/mL

application(s)

microbiology

General description

Bile bovine is extracted under controlled conditions from purified fresh bile, to be used in bacteriological culture media as a selective inhibitory agent for isolation and identification of pathogens. Bile is a yellow-green aqueous solution consisting of 70% bile salts, 22% phospholipids, 4% cholesterol, 3% proteins and 0.3% bilirubin. It is produced from cholesterol in liver hepatocytes and stored in the gallbladder. Bile acids are steroid acids found mainly in the bile of mammals and other vertebrates. They can be used as a selective agent in media for the detection of intestinal bacteria.

Application

Bile bovine has been used:
  • in De Man, Rogosa and Sharpe agar (MRS) broth for the growth test under high bile-salt condition
  • as a component in MRS to grow Lactobacillus delbrueckii subsp. lactis 200+,to study the effect of bile salts on cell morphology
  • as a component in brain heart infusion (BHI) broth for broth assays to study its effects on Enterococcus faecalis V583

Biochem/physiol Actions

Bile acids plays a physiological role in the removal of cholesterol from the body. It also participates in the intestinal solubilization and absorption of lipids.
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Ta pozycja
B8381X4875X4500
description

dried, unfractionated

description

bile acid mixture

description

Grade IV, ammonium sulfate suspension, ≥0.1 units/mg protein

description

Grade III, ammonium sulfate suspension, ≥0.8 units/mg protein

form

dried, unfractionated

form

powder

form

ammonium sulfate suspension

form

ammonium sulfate suspension

application(s)

microbiology

application(s)

-

application(s)

-

application(s)

-

sterility

non-sterile

sterility

-

sterility

-

sterility

-

solubility

water: soluble 50 mg/mL

solubility

-

solubility

-

solubility

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

color

yellow-brown

color

-

color

-

color

faint brown to dark brown


Klasa składowania

13 - Non Combustible Solids

Co się dzieje?

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves, type N95 (US)



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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  
  1. Hi, I am trying to prepare blank bile matrix using this bovine bile powder. Do you have any recommendation of mixing ratio? Thank you

    1 answer
    1. This product is a crude bile extract from ox gall bladder. B3883 is soluble in water at 50 mg per mL resulting in a turbid dark brown to green suspension. The natural concentration is 1 gram of solid material with 11.5 grams of  water. We do not have more detailed information about using it in preparing a blank matrix.

      Helpful?

  2. WHAT IS THE PROPER TEMPERATURE FOR STORAGE?

    1 answer
    1. Item B3883 can be stored at room temperature.

      Helpful?

  3. Can you tell us the exact bile salt concentration?

    1 answer
    1. This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however other components such as glycocholic and taurocholic acid are also present.

      Helpful?

  4. I need to prepare bile salt (10 mM). May I know the content of Bile in this product? Or the molecular weight of this products? Thanks.

    1 answer
    1. This product has not been purified. The bile content is not determined. The molecular weight for purified bile is listed at 408.6 g/mol, however, other components such as glycocholic and taurocholic acid are also present.

      Helpful?

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