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E1769

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

BioXtra

Synonim(y):

N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC, EDC hydrochloride, WSC hydrochloride

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Informacje o tej pozycji

Wzór empiryczny (zapis Hilla):
C8H17N3 · HCl
Numer CAS:
Masa cząsteczkowa:
191.70
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161700
EC Number:
247-361-2
MDL number:
Beilstein/REAXYS Number:
5764110
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Nazwa produktu

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, BioXtra

product line

BioXtra

Quality Segment

form

powder

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

ign. residue

≤0.1%

mp

110-115 °C (lit.)

solubility

H2O: 0.5 M, clear to very slightly hazy, colorless to very faintly yellow

anion traces

sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH4+: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%

storage temp.

−20°C

SMILES string

Cl.CCN=C=NCCCN(C)C

InChI

1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H

InChI key

FPQQSJJWHUJYPU-UHFFFAOYSA-N

Application

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:
  • for post-fixation and crosslinking of small ribonucleic acid (RNAs) species from formalin fixed paraffin embedded (FFPE) tissues, for fluorescent in situ hybridisation (FISH) and immunofluorescence (IF) signals
  • for cross-linking of nanofiber matrices with mouse lung extract
  • for activation of substrate XNA oligonucleotides

Biochem/physiol Actions

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.
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Ta pozycja
E63830345003449
description

BioXtra

description

crystalline

description

purum, ≥98.0% (AT)

description

≥99.0% (AT)

form

powder

form

crystalline

form

powder

form

powder

product line

BioXtra

product line

-

product line

-

product line

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

cation traces

Zn: ≤0.0005%, Mg: ≤0.0005%, Pb: ≤0.001%, Na: ≤0.005%, Cu: ≤0.0005%, Al: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Ca: ≤0.0005%, NH4+: ≤0.05%

cation traces

-

cation traces

-

cation traces

-

mp

110-115 °C (lit.)

mp

110-115 °C (lit.)

mp

110-115 °C

mp

112-116 °C, 110-115 °C (lit.)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


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Słowo ostrzegawcze

Danger

Narządy docelowe

Stomach,large intestine,lymph node

Klasa składowania

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

PPE (środki ochrony indywidualnej)

dust mask type N95 (US), Eyeshields, Gloves

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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