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H3136

Sigma-Aldrich

Hematoxylin

certified by the Biological Stain Commission

Synonym(s):
Natural Black 1
Empirical Formula (Hill Notation):
C16H14O6 · xH2O
CAS Number:
Molecular Weight:
302.28 (anhydrous basis)
Numer w indeksie barw:
75290
Beilstein:
91400
Numer EC:
Numer MDL:
Identyfikator substancji w PubChem:
NACRES:
NA.47

klasa czystości

certified by the Biological Stain Commission

Poziom jakości

forma

powder

mp

200 °C (dec.) (lit.)

rozpuszczalność

ethanol: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

temp. przechowywania

room temp

SMILES string

Oc1cc2C[C@@]3(O)COc4c(O)c(O)ccc4[C@H]3c2cc1O

InChI

1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1

InChI key

WZUVPPKBWHMQCE-XJKSGUPXSA-N

Gene Information

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This Item
H9627P4030198269
Hematoxylin certified by the Biological Stain Commission

Sigma-Aldrich

H3136

Hematoxylin

Hematoxylin

Supelco

H9627

Hematoxylin

Phloxine B Dye content ≥80 %, certified by the Biological Stain Commission

Sigma-Aldrich

P4030

Phloxine B

Erythrosin B certified by the Biological Stain Commission, Dye content ≥85 %

Sigma-Aldrich

198269

Erythrosin B

grade

certified by the Biological Stain Commission

grade

-

grade

certified by the Biological Stain Commission

grade

certified by the Biological Stain Commission

form

powder

form

powder

form

powder

form

powder

solubility

ethanol: 1 mg/mL

solubility

95% ethanol: 1 mg/mL

solubility

H2O: 1 mg/mL

solubility

H2O: 1 mg/mL, clear

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

mp

200 °C (dec.) (lit.)

mp

200 °C (dec.) (lit.)

mp

-

mp

315 °C

Opis ogólny

Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope.

Zastosowanie

Hematoxylin has been used:
  • to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples
  • as a nuclear counterstain to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent
  • to stain lung tissue for histological analysis

Przydatność

Certified for use in Weigert′s iron hematoxylin, Mayer′s hemalum, Harris hemalum and in Heidenhain′s technique

piktogramy

Exclamation mark

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Eye Irrit. 2

Kod klasy składowania

11 - Combustible Solids

WGK

WGK 1

Temperatura zapłonu °F

Not applicable

Temperatura zapłonu °C

Not applicable

Środki ochrony indywidualnej

dust mask type N95 (US), Eyeshields, Gloves


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