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4381663

iTRAQ® Reagent - 8PLEX Multiplex Kit

Synonim(y):

iTRAQ 8-Plex

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Informacje o tej pozycji

NACRES:
NA.24
UNSPSC Code:
12161503
Pomoc techniczna
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product line

Milliplex®

storage temp.

−20°C

General description

Contains iTRAQ® Reagents 113, 114, 115, 116, 117, 118, 119 and 121 and sufficient material for five 8-plex assays. Multiplex Buffer kit (Cat. No. 4381664) to be ordered separately.

Cat. No. 4381663 contains the equivalent reagents in the Sciex iChemistry® product number 4390812.
The isobaric tags for relative and absolute quantitation (iTRAQ®) reagents are the first set of multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples enabling simultaneous identification and quantitation, relative and absolute, while retaining important post-translational modification (PTM) information. The iTRAQ Reagents - 8plex kits are available in more economical bulk packs.

Application

iTRAQ® Reagent - 8PLEX Multiplex Kit has been used:
  • to derivatize and label the tryptic digests of the iodoacetamide probe (IPM)-labeled HeLa proteome
  • in isobaric tags for relative and absolute quantitation (iTRAQ) labeling of peptides
  • as isobaric labels for the comparative quantification of proteins between the digested samples in proteomic analysis

Features and Benefits

  • Simultaneously identify and quantify proteins from multiple samples
  • Expands protein and proteome coverage by labeling all peptides, including those with post-translational modifications (PTMs)
  • Increases confidence in identification and quantitation from MS/MS spectra by tagging multiple peptides per protein
  • Increases throughput and confidence in results for protein biomarker discovery studies
  • Offers a simple workflow without sample fractionation for reduced-complexity samples, such as affinity pull-downs
  • Provides the flexibility to multiplex up to eight different biological samples simultaneously in a single experiment
  • Fully supported by ProteinPilot Software on all SCIEX proteomics LC/MS/MS platforms

Analysis Note

To view the Protocol for the iTRAQ Reagents 8-plex Kit, please visit this Protocol link.

To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.

Legal Information

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
iChemistry is a registered trademark of AB Sciex Pte. Ltd.
iTraq is a registered trademark of AB Sciex Pte. Ltd.
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Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2

Klasa składowania

3 - Flammable liquids

flash_point_f

35.6 °F - closed cup

flash_point_c

2.0 °C - closed cup

wgk

WGK 3

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