46043

Ethidium homodimer

suitable for fluorescence, ~90% (HPCE)

• Synonim(y): EtDi, EthD-1
• Wzór empiryczny (zapis Hilla): C₄₆H₅₀Cl₄N₈
• Numer CAS: 61926-22-5
• Masa cząsteczkowa: 856.75
• NACRES: NA.32
• PubChem Substance ID: 329757072
• UNSPSC Code: 12161505
• EC Number: 263-325-9
• MDL number: MFCD00210948

Właściwości

• Quality Segment: 100
• description: suitable for the fluorogenic detection of DNA
• assay: ~90% (HPCE)
• form: solid
• mol wt: 856.75 g/mol
• concentration: ≤100% (5,5′-[Ethylenebis(iminotrimethylene)]bis[3,8-diamino-6-phenylphenanthridinium] dichloride dihydrochloride)
• technique(s): protein detection: suitable
• color: red to dark red
• mp: ≥250 °C (lit.)
• solubility: DMF: soluble, DMSO: soluble, H₂O: soluble
• fluorescence: λ_ex 493 nm in H₂O, λ_ex 528 nm; λ_em 617 nm in TE buffer; DNA
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
• InChI: 1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
• InChI key: ZKAJUKABUUJRLB-UHFFFAOYSA-N

Opis

Application

Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.

Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Reagent for the fluorimetric detection of nucleic acids

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02,GHS08,GHS05
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H261,H314,H351
• Ostrzeżenia: P231 + P232 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Klasa składowania: 4.3 - Hazardous materials which set free flammable gases upon contact with water
• Co się dzieje?: WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• PPE (środki ochrony indywidualnej): dust mask type N95 (US), Eyeshields, Gloves
• Hazard Classifications: Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - Water-react. 3