54457

HEPES

BioUltra, for molecular biology, ≥99.5% (T)

• Synonim(y): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• Numer CAS: 7365-45-9
• Masa cząsteczkowa: 238.30
• Beilstein: 883043
• Numer EC: 230-907-9
• Numer MDL: MFCD00006158
• Identyfikator substancji w PubChem: 57651473
• NACRES: NA.25

Właściwości

• klasa czystości: for molecular biology
• Poziom jakości: 200
• linia produktu: BioUltra
• Próba: ≥99.5% (T)
• forma: powder
• zanieczyszczenia: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pozostałość po prażeniu: ≤0.1% (as SO₄)
• strata: ≤0.5% loss on drying, 110 °C
• pH: 5.0-6.5 (25 °C, 1 M in H₂O)
• useful pH range: 6.8-8.2
• pKa (25°C): 7.5
• rozpuszczalność: H₂O: 1 M at 20 °C, clear, colorless
• ślady anionów: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• ślady kationów: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 1 M in H₂O
• absorpcja UV: λ: 260 nm A_max: 0.045; λ: 280 nm A_max: 0.035
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Opis

Opis ogólny

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Zastosowanie

HEPES has been used as a component of:

• DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
• RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
• Recapture solution to re-capture RNA exosomes from the glycerol gradient
• ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
• 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors

Inne uwagi

Easily compare specifications for HEPES products with the HEPES specification table.

3′-Terminal labeling of RNA with T₄ RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 13 - Non Combustible Solids
• WGK: WGK 1
• Temperatura zapłonu °F: Not applicable
• Temperatura zapłonu °C: Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)