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59747

Lactic Dehydrogenase, recombinant from E. coli

≥90 U/mg

Synonim(y):

(S)-Lactate: NAD+ oxidoreductase, L-Lactate Dehydrogenase, Lactate

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Rozmiar/SKUDostępnośćCena netto
25 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1400,00 zł
100 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
3800,00 zł

Informacje o tej pozycji

Numer CAS:
9001-60-9
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-617-8
MDL number:
MFCD00131466
Numer WE:
1.1.1.27 (BRENDA, IUBMB)
Specific activity:
≥90 U/mg
Recombinant:
expressed in E. coli

1400,00 zł

Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
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recombinant

expressed in E. coli

Quality Segment

form

powder

specific activity

≥90 U/mg

storage temp.

−20°C

General description

LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.

Application

Lactic Dehydrogenase, recombinant from E. coli has been used:
  • in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
  • to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
  • in in vitro DltC D-alanylation assay

Biochem/physiol Actions

L-lactic dehydrogenase catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.
Conversion of L-lactate into L-pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.

Other Notes

One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25°C (NADH as cofactor)
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L3888L3916SAE0049
Sigma-Aldrich

Sigma-Aldrich

59747

Lactic Dehydrogenase, recombinant from E. coli

D-Lactic Dehydrogenase from Lactobacillus leichmannii lyophilized powder, 150-500 units/mg protein

Sigma-Aldrich

L3888

D-Lactic Dehydrogenase from Lactobacillus leichmannii

L-Lactic Dehydrogenase from bovine heart 1000 units/mL

Sigma-Aldrich

L3916

L-Lactic Dehydrogenase from bovine heart

L-Lactate Dehydrogenase (LDHA) from human, recombinant, expressed in E. coli, aqueous solution

Sigma-Aldrich

SAE0049

L-Lactate Dehydrogenase (LDHA)

description

≥90 U/mg

description

lyophilized powder, 150-500 units/mg protein

description

1000 units/mL

description

from human, recombinant, expressed in E. coli, aqueous solution

specific activity

≥90 U/mg

specific activity

150-500 units/mg protein

specific activity

1000 units/mL

specific activity

-

form

powder

form

lyophilized powder

form

solution

form

aqueous solution

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

expressed in E. coli

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

Piktogramy

Health hazard
GHS08

Słowo ostrzegawcze

Danger

Kody zagrożeń

H334

Ostrzeżenia

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Klasa składowania

11 - Combustible Solids

Co się dzieje?

WGK 1

PPE (środki ochrony indywidualnej)

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  

  1. Does this enzyme catalyze the reaction of L-lactate to L-pyruvate, with NAD+ -> NADH as cofactors, or does it catalyze the conventional L-pyruvate to L-lactate with NADH -> NAD+?

    1 answer

    1. This enzyme catalyzes the conversion of L-lactate into L-pyruvate while reducing NAD+ to NADH and H+.

      Helpful?

  2. Can you provide more information on the biological source for recombinant LDH 59747? Does the certificate of origin specify whether the recombinant protein is from mice or another source? It seems that the certificate of origin does not offer detailed information to clarify this.

    1 answer

    1. The material is Lactate dehydrogenase from chicken heart, expressed in recombinant microbes.

      Helpful?

  3. I want to know the molecular weight of this protein

    1 answer

    1. This product is a recombinant enzyme from E. coli. The species and tissue of origin is the chicken heart. The molecular weight of this material has not been determined. However, according to Huston et al. 1972, Lactate Dehydrogenase from the bovine heart has a molecular weight of approximately 136,700 daltons.

      Helpful?

  4. What species is Product No. 59747, Lactic Dehydrogenase, recombinant from E. coli?

    1 answer

    1. This product is a recombinant Lactic Dehydrogenase from chicken heart made in E. coli.

      Helpful?

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